CAJ | 학술논문

目的：观察水蛭素对人牙龈成纤维细胞（HGFs）碱性成纤维细胞生长因子（bFGF）及转化生长因子-β1（TGF-β1）表达变化的规律，探讨水蛭素影响牙龈改建的可能作用机制。方法体外培养并鉴定HGFs，利用不同浓度的水蛭素分别作用于正常（对照组）和受长期机械外力作用后增生的HGFs（实验组），通过实时定量聚合酶链反应法和免疫细胞化学法检测TGF-β1及bFGF的表达。结果未加入水蛭素时，受长期机械外力作用后，实验组促进HGFs增殖胶原合成的TGF-β1表达升高，而抑制胶原合成的bFGF表达降低（P<0.05）。加入水蛭素干预增生的HGFs后，可正向调节bFGF表达，而负向调节TGF-β1的表达（P<0.05）。结论外力作用干扰了HGFs胶原合成与降解之间的平衡，水蛭素可能通过调节这一平衡而促进牙龈改建过程。
목적：관찰수질소대인아간성섬유세포（HGFs）감성성섬유세포생장인자（bFGF）급전화생장인자-β1（TGF-β1）표체변화적규률，탐토수질소영향아간개건적가능작용궤제。방법체외배양병감정HGFs，이용불동농도적수질소분별작용우정상（대조조）화수장기궤계외력작용후증생적HGFs（실험조），통과실시정량취합매련반응법화면역세포화학법검측TGF-β1급bFGF적표체。결과미가입수질소시，수장기궤계외력작용후，실험조촉진HGFs증식효원합성적TGF-β1표체승고，이억제효원합성적bFGF표체강저（P<0.05）。가입수질소간예증생적HGFs후，가정향조절bFGF표체，이부향조절TGF-β1적표체（P<0.05）。결론외력작용간우료HGFs효원합성여강해지간적평형，수질소가능통과조절저일평형이촉진아간개건과정。
Objective This study aimed to investigate the effects of hirudin on the expression of transforming growth factor (TGF-β1) and basic fibroblast growth factor (bFGF) in human gingival fibroblasts (HGFs) in vitro, as well to explore its func-tion in the mechanism of gingival remodeling. Methods After culturing was performed with classic tissue-explant method, HGFs were derived from normal gingival and gingival hyperplasia tissues followed by orthodontic treatments with different concentrations of hirudin. The mRNA and protein expression levels of TGF-β1 and bFGF were respectively detected by real time quantity polymerase chain reaction and immunocytochemistry. Results Compared with normal HGFs, TGF-β1 expres-sion promoted collagen synthesis of fibroblasts, whereas bFGF collagen synthesis was decreased in hyperplasia HGFs without hirudin (P<0.05). Hirudin significantly upregulated the expression levels of bFGF but downregulated TGF-β1 in hyperplasia HGFs (P<0.05). Conclusion Orthodontic force may influence the balance of collagen synthesis and degradation in HGFs. Hirudin may modulate the balance of HGF collagen metabolism, thereby promoting gingival remodeling.