华西口腔医学杂志
華西口腔醫學雜誌
화서구강의학잡지
WEST CHINA JOURNAL OF STOMATOLOGY
2015年
1期
88-92
,共5页
李红%纪海%何艳艳%杨圣辉%侯本祥
李紅%紀海%何豔豔%楊聖輝%侯本祥
리홍%기해%하염염%양골휘%후본상
牙髓卟啉单胞菌%原发性感染根管%再感染根管%慢性根尖周炎%实时荧光定量聚合酶链反应
牙髓卟啉單胞菌%原髮性感染根管%再感染根管%慢性根尖週炎%實時熒光定量聚閤酶鏈反應
아수계람단포균%원발성감염근관%재감염근관%만성근첨주염%실시형광정량취합매련반응
Porphyromonas endodontalis%primary endodontic infection%secondary endodontic infection%chronic apical periodontitis%real-time fluorescence quantitative polymerase chain reaction
目的：采用16s rDNA PCR和实时荧光定量聚合酶链反应（RTFQ-PCR）分析和比较牙髓卟啉单胞菌（P. en-dodontalis）在原发性感染和再感染根管中的定植情况。方法将120例慢性根尖周炎患者的120颗单根管患牙按原发性感染和再感染分为2组，每组60颗。采用16s rDNA PCR分析P. endodontalis在两种感染根管内的检出率，对于P. en-dodontalis检出阳性者用RTFQ-PCR比较P. endodontalis在两种感染根管内的DNA相对表达量。结果 P. endodontalis在原发性感染根管内的检出率明显高于再感染根管的检出率（P=0.001）。RTFQ-PCR检测结果表明P. endodontalis在原发性感染根管内的DNA表达量和再感染根管内DNA表达量差异无统计学意义（P=0.303）。结论 P.endodontalis在原发性感染和再感染根管内均有定植，但与原发性感染根管关系更为密切。
目的：採用16s rDNA PCR和實時熒光定量聚閤酶鏈反應（RTFQ-PCR）分析和比較牙髓卟啉單胞菌（P. en-dodontalis）在原髮性感染和再感染根管中的定植情況。方法將120例慢性根尖週炎患者的120顆單根管患牙按原髮性感染和再感染分為2組，每組60顆。採用16s rDNA PCR分析P. endodontalis在兩種感染根管內的檢齣率，對于P. en-dodontalis檢齣暘性者用RTFQ-PCR比較P. endodontalis在兩種感染根管內的DNA相對錶達量。結果 P. endodontalis在原髮性感染根管內的檢齣率明顯高于再感染根管的檢齣率（P=0.001）。RTFQ-PCR檢測結果錶明P. endodontalis在原髮性感染根管內的DNA錶達量和再感染根管內DNA錶達量差異無統計學意義（P=0.303）。結論 P.endodontalis在原髮性感染和再感染根管內均有定植，但與原髮性感染根管關繫更為密切。
목적：채용16s rDNA PCR화실시형광정량취합매련반응（RTFQ-PCR）분석화비교아수계람단포균（P. en-dodontalis）재원발성감염화재감염근관중적정식정황。방법장120례만성근첨주염환자적120과단근관환아안원발성감염화재감염분위2조，매조60과。채용16s rDNA PCR분석P. endodontalis재량충감염근관내적검출솔，대우P. en-dodontalis검출양성자용RTFQ-PCR비교P. endodontalis재량충감염근관내적DNA상대표체량。결과 P. endodontalis재원발성감염근관내적검출솔명현고우재감염근관적검출솔（P=0.001）。RTFQ-PCR검측결과표명P. endodontalis재원발성감염근관내적DNA표체량화재감염근관내DNA표체량차이무통계학의의（P=0.303）。결론 P.endodontalis재원발성감염화재감염근관내균유정식，단여원발성감염근관관계경위밀절。
Objective This study aims to assess and compare the prevalence of Porphyromonas endodontalis (P. endo-dontalis) in root canals associated with primary and secondary endodontic infections by using 16s rDNA PCR and real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR). Methods A total of 120 adult patients with one radio-graphically documented periapical lesion were included. Sixty teeth presented with primary endodontic infections and 60 with secondary endodontic infections requiring retreatment. P. endodontalis was identified by using 16s rDNA PCR techni-ques. The positive DNA expression of P. endodontalis in two types of infected root canals were quantitatively compared by using SYBR GREEN Ⅰ RTFQ-PCR. Results The prevalence of P. endodontalis in the root canals with primary endodontic infections was significantly higher than that in root canals with secondary endodontic infections (P=0.001). However, RTFQ-PCR results showed no significant difference in DNA expression quantities between the primary and secondary endodontic infections root canals (P=0.303). Conclusion P. endodontalis is more highly associated with root canals having primary endodontic infections, although P. endodontalis colonize in both root canals with primary and secondary chronic apical periodontitis.
