华西口腔医学杂志
華西口腔醫學雜誌
화서구강의학잡지
WEST CHINA JOURNAL OF STOMATOLOGY
2015年
1期
80-83
,共4页
刘敏%闫嘉惟%刘娅玲%郝玉庆
劉敏%閆嘉惟%劉婭玲%郝玉慶
류민%염가유%류아령%학옥경
格氏链球菌%atlS基因%荧光定量聚合酶链反应
格氏鏈毬菌%atlS基因%熒光定量聚閤酶鏈反應
격씨련구균%atlS기인%형광정량취합매련반응
Streptococcus gordonii%atlS gene%fluorescence quantitative polymerase chain reaction
目的：检测格氏链球菌自溶酶atlS基因在不同生长期和pH值条件下表达水平的差异，分析生长期和pH值对格氏链球菌atlS基因表达的影响，进而分析调控格氏链球菌atlS基因表达的因素。方法收集不同生长期（对数生长早期、对数生长中期、对数生长晚期、稳定期）及不同pH值（7.0和5.5）条件下培养的格氏链球菌野生株ATCC 35105细胞，常规方法提取总RNA。采用荧光定量聚合酶链反应（FQ-PCR）法，以细菌的16S rRNA为内参照，分别测定atlS基因mRNA的相对表达量，比较格氏链球菌在不同生长期及pH值下atlS mRNA的表达水平。结果 FQ-PCR结果发现，atlS基因表达从对数生长早期到稳定期逐步升高，中性条件下atlS基因表达高于酸性条件，其差异有统计学意义（P<0.05）。结论格氏链球菌atlS基因表达受细菌生长期和pH值的影响。
目的：檢測格氏鏈毬菌自溶酶atlS基因在不同生長期和pH值條件下錶達水平的差異，分析生長期和pH值對格氏鏈毬菌atlS基因錶達的影響，進而分析調控格氏鏈毬菌atlS基因錶達的因素。方法收集不同生長期（對數生長早期、對數生長中期、對數生長晚期、穩定期）及不同pH值（7.0和5.5）條件下培養的格氏鏈毬菌野生株ATCC 35105細胞，常規方法提取總RNA。採用熒光定量聚閤酶鏈反應（FQ-PCR）法，以細菌的16S rRNA為內參照，分彆測定atlS基因mRNA的相對錶達量，比較格氏鏈毬菌在不同生長期及pH值下atlS mRNA的錶達水平。結果 FQ-PCR結果髮現，atlS基因錶達從對數生長早期到穩定期逐步升高，中性條件下atlS基因錶達高于痠性條件，其差異有統計學意義（P<0.05）。結論格氏鏈毬菌atlS基因錶達受細菌生長期和pH值的影響。
목적：검측격씨련구균자용매atlS기인재불동생장기화pH치조건하표체수평적차이，분석생장기화pH치대격씨련구균atlS기인표체적영향，진이분석조공격씨련구균atlS기인표체적인소。방법수집불동생장기（대수생장조기、대수생장중기、대수생장만기、은정기）급불동pH치（7.0화5.5）조건하배양적격씨련구균야생주ATCC 35105세포，상규방법제취총RNA。채용형광정량취합매련반응（FQ-PCR）법，이세균적16S rRNA위내삼조，분별측정atlS기인mRNA적상대표체량，비교격씨련구균재불동생장기급pH치하atlS mRNA적표체수평。결과 FQ-PCR결과발현，atlS기인표체종대수생장조기도은정기축보승고，중성조건하atlS기인표체고우산성조건，기차이유통계학의의（P<0.05）。결론격씨련구균atlS기인표체수세균생장기화pH치적영향。
Objective This study aimed to detect the difference in the expression levels of autolysin atlS gene of Strepto-coccus gordonii (S. gordonii) at different growth stages and pH values, as well as to analyze the factors regulating atlS gene expression in S. gordonii. Methods S. gordonii wild strains (ATCC 35105) were collected at different growth stages (early exponential phase, mid-exponential phase, late exponential stage, and platform stage) and pH values (pH 7 and pH 5.5), and total RNA was extracted by using a conventional method. Fluorescence quantitative polymerase chain reaction (FQ-PCR) was used to measure the relative mRNA expression of atlS gene, with bacterial 16S rRNA as internal reference, for a com-parison of the mRNA levels of atlS gene expression in S.gordonii at different growth stages and pH values. Results FQ-PCR results showed that atlS gene expression increased with gradually increasing growth stage under neutral conditions and was higher than that under acidic conditions (P<0.05). Conclusion The atlS gene expression in S.gordonii is influenced by growth stage and pH value factors.
