CAJ | 학술논문

目的：探讨快速原型制作纳米-羟基磷灰石/聚己内酯（nano-HA/PCL）根形三维支架的细胞生物相容性，为进一步体内实验提供研究基础。方法：通过CT 扫描获得犬头颅骨影像信息，以CAD 软件实现犬下颌牙根形态的三维重建影像，应用快速原型（RP）技术制作nano-HA/PCL根形支架，经犬骨髓基质细胞接种后体外复合培养，检测支架材料的细胞相容性。结果：通过CT影像和快速原型技术，可实现解剖根形态结构nano-HA/PCL三维仿真支架。细胞支架复合培养后，扫描电镜显示细胞生长附着于支架表面；细胞与支架复合后增殖情况良好；7 d和14 d碱性磷酸酶活性高于对照组。结论：体外复合培养结果表明，RP技术制备的nano-HA/PCL支架和骨髓基质细胞生物相容性较好，可进一步应用于牙槽骨缺损骨组织工程修复动物实验。
목적：탐토쾌속원형제작납미-간기린회석/취기내지（nano-HA/PCL）근형삼유지가적세포생물상용성，위진일보체내실험제공연구기출。방법：통과CT 소묘획득견두로골영상신식，이CAD 연건실현견하합아근형태적삼유중건영상，응용쾌속원형（RP）기술제작nano-HA/PCL근형지가，경견골수기질세포접충후체외복합배양，검측지가재료적세포상용성。결과：통과CT영상화쾌속원형기술，가실현해부근형태결구nano-HA/PCL삼유방진지가。세포지가복합배양후，소묘전경현시세포생장부착우지가표면；세포여지가복합후증식정황량호；7 d화14 d감성린산매활성고우대조조。결론：체외복합배양결과표명，RP기술제비적nano-HA/PCL지가화골수기질세포생물상용성교호，가진일보응용우아조골결손골조직공정수복동물실험。
Objective:To fabricate nano-hydroxyapatite/Poly-ε-caprolactone(nano-HA/PCL) scaffold with three-dimensional simulation of the root shape by using a rapid prototyping (RP) process. To evaluate biocompatibility of the scaffold used for bone tissue engineering. Methods: Parietal bone image information of canine was obtained by CT scan, three-dimensional reconstruction images were performed by CAD, roots shape nano-HA/PCL scaffolds was obtained by RP technology. Canine 3rd passage bone marrow stromal cells (BMSCs) were seeded in nano-HA/PCL scaffolds and grown in osteogenic medium. Cells were harvested after 1, 3, and 7 days. BMSCs proliferation and ALP activity were measured. Results: Scanning electron micrographs showed the BMSCs adhered to the inner surface of the scaffolds. Cell proliferation steadily increased with time, and alkaline phosphatase activity in the scaffolds at 7- day and 14- day were enhanced. Conclusion: The os-teogenic potential of BMSCs seeded at the rapid prototyped nano-HA/PCL scaffolds demonstrate a good biocompatibility, and may be effective for animal experiment in alveolar bone repair in the near future.