CAJ | 학술논문

目的：：评价雌激素受体α( estrogen receptor α, ERα)基因XbaⅠ酶切片段多态性( XbaⅠ多态性)、膳食大豆摄入与乳腺癌患病风险的关系。方法：采用病例对照研究设计,序贯收集病例对照各291例。采用问卷调查收集乳腺癌常见危险因素信息；采用频次调查及单次摄入量调查,收集膳食大豆类食物摄入情况,并转换为大豆异黄酮日摄入量。采用限制性片段长度多态性技术,检测XbaⅠ(rs9340799)A→G(x→X)基因突变。采用多因素Logistic回归分析XbaⅠ多态性、膳食大豆摄入的主效应和交互效应,并计算相乘交互系数。采用delta原理计算相加交互系数及其可信区间。结果：在总人群及绝经前后亚组中,携带X突变等位基因与乳腺癌患病风险无关( Xx vs. xx：OR=0.61~0.82；XX vs. xx：OR=0.60~3.62； Xx+XX vs. xx： OR=0.61~0.92,95%CI均包括1)。膳食大豆高摄入对乳腺癌具有保护作用(总人群：OR=0.64,95%CI：0.42~0.97；绝经后：OR=0.40,95%CI：0．19~0.81)。总人群中,携带X等位基因且膳食大豆高摄入进一步降低乳腺癌风险( OR=0.47,95%CI：0.25~0．89)。两者相乘交互系数IOR=1.15,95%CI：0.49~2.76；相加交互系数RERI=0.19,95%CI：-0.35~0.73；API=0.41,95%CI：-0.75~1.57；S=0.73,95%CI：0.34~1.59。结论：膳食大豆摄入可能会降低乳腺癌风险,但ERα基因XbaⅠ酶切片段多态性位点X等位基因与膳食大豆高摄入之间是否存在交互作用尚需进一步探讨。
목적：：평개자격소수체α( estrogen receptor α, ERα)기인XbaⅠ매절편단다태성( XbaⅠ다태성)、선식대두섭입여유선암환병풍험적관계。방법：채용병례대조연구설계,서관수집병례대조각291례。채용문권조사수집유선암상견위험인소신식；채용빈차조사급단차섭입량조사,수집선식대두류식물섭입정황,병전환위대두이황동일섭입량。채용한제성편단장도다태성기술,검측XbaⅠ(rs9340799)A→G(x→X)기인돌변。채용다인소Logistic회귀분석XbaⅠ다태성、선식대두섭입적주효응화교호효응,병계산상승교호계수。채용delta원리계산상가교호계수급기가신구간。결과：재총인군급절경전후아조중,휴대X돌변등위기인여유선암환병풍험무관( Xx vs. xx：OR=0.61~0.82；XX vs. xx：OR=0.60~3.62； Xx+XX vs. xx： OR=0.61~0.92,95%CI균포괄1)。선식대두고섭입대유선암구유보호작용(총인군：OR=0.64,95%CI：0.42~0.97；절경후：OR=0.40,95%CI：0．19~0.81)。총인군중,휴대X등위기인차선식대두고섭입진일보강저유선암풍험( OR=0.47,95%CI：0.25~0．89)。량자상승교호계수IOR=1.15,95%CI：0.49~2.76；상가교호계수RERI=0.19,95%CI：-0.35~0.73；API=0.41,95%CI：-0.75~1.57；S=0.73,95%CI：0.34~1.59。결론：선식대두섭입가능회강저유선암풍험,단ERα기인XbaⅠ매절편단다태성위점X등위기인여선식대두고섭입지간시부존재교호작용상수진일보탐토。
Objective: To assess the association between estrogen receptor α genes XbaI polymorphisms, dietary soy intake and breast cancer. Methods:A case-control study was designed. A total of 291 pairs were sequentially collect-ed. Questionnaires were used to obtain the common risk factors of breast cancer. Daily intake of soy isoflavone ( DISI) was collected by food frequency survey combined with investigation of every intake. Restricted fragment length polymorphisms were used to examine XbaⅠ(rs9340799) A→G (x→X). Multiple logistic regression model was used to evaluate the sin-gle and interactive effects of XbaⅠpolymorphism and DISI. Multiplication interaction coefficient was also calculated. Based on the delta theory, the interaction coefficients of additive model with 95% CI were calculated. Results:There was no as-sociation between XbaⅠpolymorphism and breast cancer among general women and menopausal subgroups( Xx vs. xx:OR= 0. 61~0. 82;XX vs. xx:OR=0. 60~3. 62;Xx+XX vs. xx:OR= 0. 61~0. 92, all the 95%CIs included 1). High multiplication interaction coefficient IOR=1. 15, 95% CI: 0. 49 ~2. 76. The interaction coefficients of additive model were as follows:RERI=0. 19, 95%CI: -0. 35~0. 73;API=0. 41, 95% CI: -0. 75~1. 57;S=0. 73, 95% CI:0. 34~1. 59. Conclusion: High intake of soy could reduce breast cancer risk. However, the interaction between estrogen receptor α genes XbaI polymorphisms and soy intake needs to be further explored.