CAJ | 학술논문

茶多酚（tea polyphenols， TP）是一种具有广谱抗菌作用的活性质，以大肠杆菌（Escherichia coli， E．coli）为研究对象，采用牛津杯实验研究茶多酚的最小抑菌浓度，并采用结晶紫实验、电导率测定、离子泄漏测定来分析TP对E．coli细胞膜通透性的影响。通过琼脂糖凝胶电泳分析TP对E．coli DNA的影响。结果显示，TP对E．coli的最小抑菌浓度为40μg ／mL；结晶紫实验中，随着浓度的增大，OD590也随之增大；随着处理时间的增长，电导率随之增大，离子泄漏随之增多，说明TP可以作用于E．coli的细胞膜，能够改变其通透性。通过琼脂糖凝胶电泳分析可知TP处理后和空白对照组相比较，DNA条带出现变暗、拖尾现象，说明TP能够作用于E．coli的遗传物质DNA。
다다분（tea polyphenols， TP）시일충구유엄보항균작용적활성질，이대장간균（Escherichia coli， E．coli）위연구대상，채용우진배실험연구다다분적최소억균농도，병채용결정자실험、전도솔측정、리자설루측정래분석TP대E．coli세포막통투성적영향。통과경지당응효전영분석TP대E．coli DNA적영향。결과현시，TP대E．coli적최소억균농도위40μg ／mL；결정자실험중，수착농도적증대，OD590야수지증대；수착처리시간적증장，전도솔수지증대，리자설루수지증다，설명TP가이작용우E．coli적세포막，능구개변기통투성。통과경지당응효전영분석가지TP처리후화공백대조조상비교，DNA조대출현변암、타미현상，설명TP능구작용우E．coli적유전물질DNA。
Tea polyphenols is an active material with broad-spectrum antibacterial action .In this paper , Oxford cup experiment was performed to study the minimal inhibitory concentration of TP on E.coli.Crystal violet assay , electrical conductivity test and ion leak-age measurement were also performed to determine the effect of TP on cell membrane of E.coli.Agarose gel electrophoresis was carried out to analyze the effect of TP on E.coli DNA.The results was the minimal inhibitory concentration of TP was 40 μg/mL.The value of OD590 increased with the increase of TP concentration .And the value of conductivity and ion leakage increased with the increase of time, which shown that TP could change cell membrane permeability .DNA of E.coli treated with TP was extracted and compared with blank control group , the band of DNA of experiment group emerged dimmed and trailing , and this result indicated that TP can act on DNA of E.coli.