CAJ | 학술논문

为了缓解红豆杉种质资源匮乏的现状和提高人工种植的红豆杉品质，试验运用水培技术，利用赤霉素（ GA3）处理诱导水培红豆杉生根，通过比较水培与土培中国红豆杉在根系结构、叶片色素含量、超氧化物歧化酶（SOD）活性、过氧化氢酶（CAT）活性、过氧化物酶（POD）、丙二醛（MDA）含量等部分指标的区别，揭示水培对红豆杉根系结构及生理生化的影响，探索从土培到水培转化过程中的变化规律。结果表明：400 mg／L GA3处理能显著促进水生根的形成；水培红豆杉洁白光滑，侧根少，无根毛，在显微结构上，细胞紧凑，缺少维管柱细胞，有发达的通气组织；水培红豆杉叶绿素含量低于土培组，但SOD、CAT、POD活性均高于土培组（ P＜0．01），MDA含量无显著区别，表明红豆杉从土培到水培转变过程中，部分形态及生理生化指标会发生改变来适应新环境，说明水培可以影响红豆杉的根系结构、色素含量以及部分抗氧化能力。实验为红豆杉的大规模无土栽培提供科学的理论依据。
위료완해홍두삼충질자원궤핍적현상화제고인공충식적홍두삼품질，시험운용수배기술，이용적매소（ GA3）처리유도수배홍두삼생근，통과비교수배여토배중국홍두삼재근계결구、협편색소함량、초양화물기화매（SOD）활성、과양화경매（CAT）활성、과양화물매（POD）、병이철（MDA）함량등부분지표적구별，게시수배대홍두삼근계결구급생리생화적영향，탐색종토배도수배전화과정중적변화규률。결과표명：400 mg／L GA3처리능현저촉진수생근적형성；수배홍두삼길백광활，측근소，무근모，재현미결구상，세포긴주，결소유관주세포，유발체적통기조직；수배홍두삼협록소함량저우토배조，단SOD、CAT、POD활성균고우토배조（ P＜0．01），MDA함량무현저구별，표명홍두삼종토배도수배전변과정중，부분형태급생리생화지표회발생개변래괄응신배경，설명수배가이영향홍두삼적근계결구、색소함량이급부분항양화능력。실험위홍두삼적대규모무토재배제공과학적이론의거。
In order to alleviate the current situation of Taxus chinensis germplasm resource scarcity and improve the quality of artificial planting Taxus chinensis, the gibberellic acid ( GA3 ) to treat the biennial yew Taxus chinensis was used to establish a inducing system of water root.Root structure, leaf pigment content and part enzyme activities , such as siperoxide dismutase(SOD), hydrogen peroxide (CAT), peroxidase(POD), malondialdehyde content(MDA), were measured in comparison of hydroponic and soil cultured yews to reveal the impact of hydroponic on Taxus chinensis root structure physiologically and biochemically and explore the changing rules from soil culture to hydroponic of Taxus chinensis.The optimal inducing concentration of GA 3 promoting yew rooting of water root was 400 mg/L.The hydroponic yew root structure was smoother and more white and compact than those in soil .Developed aerenchyma organi-zation and lack of vascular cylinder cell and root hair and less lateral root were observed in hydroponic group .The contents of pigment in hydroponic group were lower than that in soil cultured group .The activity of SOD , CAT and POD in hydroponic group was higher than that in soil cultured group ( P<0.01 ) , although the MDA content showed no significant change .Our studies suggest that part of the morphological and physiological and biochemical indexes were changed to adapt to low oxygen environment during the hydroponic process, showing hydroponic can influence of root structure , pigment contents and some of the capacity of antioxidant , providing scien-tific theory basis for large fields of soilless cultivation of Taxus chinensis.