CAJ | 학술논문

HPLC测定水稻叶中维生素C （ Vc）含量的测定方法，测定后比较转基因水稻叶与普通水稻叶中Vc含量的差异，为寻找调控水稻中Vc含量的关键基因奠定基础。草酸水溶液提取水稻叶中的Vc后，反相HPLC方法测定含量，选用的色谱柱为Diamonsil C18柱（250×4．6 mm，5μm），流动相为0．2％磷酸－甲醇，梯度洗脱，检测波长245 nm，柱温30℃。测的线性范围为1～40μg／mL（r＝0．9992），平均回收率为98．51％，转基因水稻叶组含量为（0．356±0．009）和（0．444±0．008） mg／g，而普通组Vc含量为（0．554±0．013） mg／g，统计软件SPSS19．0分析表明二者有显著差异（ P＜0．01）。此方法测水稻叶中Vc含量具有简便，快速，准确的特点；类受体蛋白激酶439即为影响水稻中Vc含量的关键基因。
HPLC측정수도협중유생소C （ Vc）함량적측정방법，측정후비교전기인수도협여보통수도협중Vc함량적차이，위심조조공수도중Vc함량적관건기인전정기출。초산수용액제취수도협중적Vc후，반상HPLC방법측정함량，선용적색보주위Diamonsil C18주（250×4．6 mm，5μm），류동상위0．2％린산－갑순，제도세탈，검측파장245 nm，주온30℃。측적선성범위위1～40μg／mL（r＝0．9992），평균회수솔위98．51％，전기인수도협조함량위（0．356±0．009）화（0．444±0．008） mg／g，이보통조Vc함량위（0．554±0．013） mg／g，통계연건SPSS19．0분석표명이자유현저차이（ P＜0．01）。차방법측수도협중Vc함량구유간편，쾌속，준학적특점；류수체단백격매439즉위영향수도중Vc함량적관건기인。
The difference of Vc content in leaves was compared between transgenic rice and ordinary rice to find the key genes which regulate the Vc content .Leaf Vc in the rice leaves was extracted with oxalic acid solution .The determination was performed with RP-HPLC on Diamonsil C18 column(250 ×4.6 mm,5μm) under the condition of gradient elution with 0.2%phosphoric acid-methanol as mobile phase , detection wavelength of 245 nm and column temperature at 30℃.The detection linear range was 1-40 μg/mL ( r=0.9992) and average recovery was 98.51%.The statistic analysis with SPSS19.0 soft showed significant differences (P<0.01) of Vc content between transgenic rice leaf group, (0.356 ±0.009) and (0.444 ±0.008) mg/g, and the normal group, (0.554 ±0.013) mg/g.Result showed that this method was simple , rapid and accurate for determination of Vc in rice leaf .The result indicated that re-ceptor protein 439 was the key gene for regulation on Vc content in the rice .