CAJ | 학술논문

为探明猕猴桃溃疡病病菌漆酶基因的序列特征与功能，研究从12株溃疡病病菌丁香假单孢杆菌猕猴桃致病变种（ Pseudomonas syringae pv．actinidae ）中扩增获得漆酶基因，分别命名为 PsalacJF、PsalacHY、PsalacGC、PsalacHWD、PsalacINS、Psalac7285、PsalacLT12、PsalacLT16、PsalacLT26、Psalac349、PsalacKw30和PsalacK3。序列分析结果显示上述序列之间只有少许核酸位点有差异，基因编码全长都为1374 bp，BLAST预测基因编码有457个氨基酸序列（包括N－端20个氨基酸的信号肽），启动子位于基因上游175 bp。生物信息学分析发现该基因具有漆酶特有的4个Cu2＋活性位点，且活性位点高度保守。系统进化分析结果显示该基因编码序列与多种细菌的漆酶基因同源关系很近，其中与P．fluorescens Pf0－1的同源性最高。研究结果表明溃疡病病菌中普遍含有具备细菌漆酶基因家族的序列特性，推测该基因可能参与调控溃疡病病菌致病性和Cu2＋耐受性。
위탐명미후도궤양병병균칠매기인적서렬특정여공능，연구종12주궤양병병균정향가단포간균미후도치병변충（ Pseudomonas syringae pv．actinidae ）중확증획득칠매기인，분별명명위 PsalacJF、PsalacHY、PsalacGC、PsalacHWD、PsalacINS、Psalac7285、PsalacLT12、PsalacLT16、PsalacLT26、Psalac349、PsalacKw30화PsalacK3。서렬분석결과현시상술서렬지간지유소허핵산위점유차이，기인편마전장도위1374 bp，BLAST예측기인편마유457개안기산서렬（포괄N－단20개안기산적신호태），계동자위우기인상유175 bp。생물신식학분석발현해기인구유칠매특유적4개Cu2＋활성위점，차활성위점고도보수。계통진화분석결과현시해기인편마서렬여다충세균적칠매기인동원관계흔근，기중여P．fluorescens Pf0－1적동원성최고。연구결과표명궤양병병균중보편함유구비세균칠매기인가족적서렬특성，추측해기인가능삼여조공궤양병병균치병성화Cu2＋내수성。
The objective of this study was to reveal sequence features of laccase , which would be used in further study of pathogenesis of kiwifruit bacterial canker caused by Pseudomonas syringae pv.actinidae.12 laccase genes homologs PsalacJF, PsalacHY, Psa-lacGC, PsalacHWD, PsalacINS, Psalac7285, PsalacLT12, PsalacLT16, PsalacLT26, Psalac349, PsalacKw30 and PsalacK3 were cloned from different Pseudomonas syringae pv.actinidae strains isolated from diverse regions and countries .The results showed that the complete DNA sequence of laccase gene were 1374 bp in length, encoding a putative proteins of 457-amino acid.All canker strains share the same amino acid sequences and small nucleic acid sequence differences , containing four highly conservative Cu 2+active sites.Promoter is located in the upstream gene with 175 bp.Phylogenetic analysis indicated that putative laccase sequence was homolo -gous to P.fluorescens Pf0-1 laccase CP000094.2 .The studies suggested that laccase gene had the sequence characteristics of laccase gene family in bacteria , which meant that laccase might involve in the pathogenicity and copper-resistant of Pseudomonas syringae pv. actinidae.