现代检验医学杂志
現代檢驗醫學雜誌
현대검험의학잡지
JOURNAL OF MODERN LABORATORY MEDICINE
2015年
1期
42-45
,共4页
幽门螺杆菌%功能性oipA%cagA%消化性疾病
幽門螺桿菌%功能性oipA%cagA%消化性疾病
유문라간균%공능성oipA%cagA%소화성질병
Helicobacter pylori%functional oipA gene%cagA%digestive disease
目的:评价幽门螺杆菌(Hp)oipA与消化系统疾病的关系。方法收集活检胃组织标本360份,对组织快速尿素酶实验阳性和部分阴性的活检标本进行 Hp分离培养,分离菌经革兰氏染色镜检、尿素酶实验和16SrRNA PCR鉴定后, PCR扩增其oipA和 cagA基因,测序分析oipA信号区的开关状态,分析功能性 oipA与临床疾病之间的关联性。结果组织标本分离培养获得106株 Hp,其中72株 cagA基因阳性,87株oipA信号区基因阳性,经测序分析80株 oipA基因信号区呈开放状态,1株oipA基因信号区呈关闭状态,6株开关状态不能确定;在溃疡和萎缩性胃炎中,功能性 oipA的检出率均显著高于 cagA。结论功能性oipA与严重消化系统疾病关系密切。
目的:評價幽門螺桿菌(Hp)oipA與消化繫統疾病的關繫。方法收集活檢胃組織標本360份,對組織快速尿素酶實驗暘性和部分陰性的活檢標本進行 Hp分離培養,分離菌經革蘭氏染色鏡檢、尿素酶實驗和16SrRNA PCR鑒定後, PCR擴增其oipA和 cagA基因,測序分析oipA信號區的開關狀態,分析功能性 oipA與臨床疾病之間的關聯性。結果組織標本分離培養穫得106株 Hp,其中72株 cagA基因暘性,87株oipA信號區基因暘性,經測序分析80株 oipA基因信號區呈開放狀態,1株oipA基因信號區呈關閉狀態,6株開關狀態不能確定;在潰瘍和萎縮性胃炎中,功能性 oipA的檢齣率均顯著高于 cagA。結論功能性oipA與嚴重消化繫統疾病關繫密切。
목적:평개유문라간균(Hp)oipA여소화계통질병적관계。방법수집활검위조직표본360빈,대조직쾌속뇨소매실험양성화부분음성적활검표본진행 Hp분리배양,분리균경혁란씨염색경검、뇨소매실험화16SrRNA PCR감정후, PCR확증기oipA화 cagA기인,측서분석oipA신호구적개관상태,분석공능성 oipA여림상질병지간적관련성。결과조직표본분리배양획득106주 Hp,기중72주 cagA기인양성,87주oipA신호구기인양성,경측서분석80주 oipA기인신호구정개방상태,1주oipA기인신호구정관폐상태,6주개관상태불능학정;재궤양화위축성위염중,공능성 oipA적검출솔균현저고우 cagA。결론공능성oipA여엄중소화계통질병관계밀절。
Objective To evaluate the relationship between functional oipA gene of Helicobacterpylori and digestive disease. Methods Biopsy gastric mucosa were obtained from 360 patients who would get gastroscopy.Hp were isolated from urease positive samples and partly urease negative samples,and improved by microscope,urease experiment and 16SrRNA PCR. OipA and cagA of the isolates were obtained by PCR and the statas of oipA signal region were analysised after sequencing. Analyze the relevance between functional oipA gene of Helicobacter pylori and digestive disease.Results 106 isolated Hp were obtained,in which 72 strains with cagA gene positive,87 with oipA signal region gene positive (80 the statas of signal region was on and 1 was off;6 could not be decided).The frequency of functional oipA were both significantly higher than cagA in ulcer and atrophic gastritis.Conclusion The relationship between functional oipA gene and digestive disease was more closer than that of cagA.