CAJ | 학술논문

目的：探讨铜绿假单胞菌对喹诺酮类药物的耐药质粒与周围共生菌耐药质粒染色体相互转移的关系。方法收集深圳市第七人民医院2011年1月～2013年12月481份各种样品培养出的铜绿假单胞菌31例，药敏实验结果对喹诺酮耐药的15例为研究对象，运用质粒转化、提取实验证实其耐药质粒的存在，PCR扩增、基因序列分析，与周围共生菌耐药质粒的基因序列比对。结果铜绿假单胞菌耐药株的耐药质粒与周围共生菌质粒发现有相同基因序列（χ2检验，χ2＝1.207，P＜0.01）。结论耐药质粒能在不同种属细菌之间传递。
목적：탐토동록가단포균대규낙동류약물적내약질립여주위공생균내약질립염색체상호전이적관계。방법수집심수시제칠인민의원2011년1월～2013년12월481빈각충양품배양출적동록가단포균31례，약민실험결과대규낙동내약적15례위연구대상，운용질립전화、제취실험증실기내약질립적존재，PCR확증、기인서렬분석，여주위공생균내약질립적기인서렬비대。결과동록가단포균내약주적내약질립여주위공생균질립발현유상동기인서렬（χ2검험，χ2＝1.207，P＜0.01）。결론내약질립능재불동충속세균지간전체。
Objective Pseudomonasaeruginosa resistance to quinolones and around symbiotic bacteria resistant plasmids each chromosome metastasis.Methods 481 samples was collected from the Seventh People’s Hospital of Shenzhen since January 2011 to December 2013,and it cultured Pseudomonasaeruginosa 31 cases,susceptibility testing confirmed 15 cases of quin-olone-resistant as the research obj ect,using plasmid transformation,pick up experiments confirmed the presence of the re-sistance plasmid,PCR amplification,gene sequence analysis,and gene sequences surrounding the symbiotic bacteria resistant plasmids as a same.Results The same gene sequence of plasmid was found between drug-resistant strains of Pseudomonas aeruginosa and the surrounding symbiotic bacteria[χ2=1.207,P<0.01].Conclusion Resistance plasmids could be trans-ferred between different species of bacteria.