现代检验医学杂志
現代檢驗醫學雜誌
현대검험의학잡지
JOURNAL OF MODERN LABORATORY MEDICINE
2015年
1期
23-26
,共4页
慢性丙型肝炎%外周血单个核细胞%Toll样受体%实时荧光定量PCR
慢性丙型肝炎%外週血單箇覈細胞%Toll樣受體%實時熒光定量PCR
만성병형간염%외주혈단개핵세포%Toll양수체%실시형광정량PCR
chronic hepatitis C%peripheral blood mononuclear cells%toll like receptor%real-time quantitative PCR
目的:观察不同性别和年龄慢性丙型肝炎(CHC)患者外周血单个核细胞(PBMC)中Toll样受体3(TLR3)和 Toll样受体7(TLR7)mRNA表达,探讨性别和年龄在CHC患者外周血TLR3和TLR7基因表达中的影响。方法选择 CHC患者115例,健康人群113例,取抗凝全血,实时荧光定量PCR检测TLR3和TLR7 mRNA表达。结果 CHC患者TLR3和TLR7表达量与健康人群相比,差异均有统计学意义(t=38.73,6.16,P<0.05)。CHC患者中非绝经期女性 TLR3和TLR7表达量与绝经期女性和青年男性相比,差异均有统计学意义(t=61.210,6.464,P<0.05;t=24.166,26.266,P<0.05)。CHC患者中老年男性 TLR3和 TLR7表达量与青年男性相比,差异均有统计学意义(t=86.349,19.583,P<0.05),而与绝经期女性相比,仅TLR3表达量差异有统计学意义(t=122.941,P<0.05)。不同性别和年龄 CHC患者的TLR3,TLR7基因表达量与 HCV-RNA载量均无相关性(|r|<0.40,P>0.05),但是青年女性 CHC患者 HCV含量均比青年男性和老年女性低,差异有统计学意义(t=3.49,2.51,P<0.05),老年男性患者 HCV含量比青年男性低,差异有统计学意义(t=2.35,P<0.05),老年男性和女性 CHC患者 HCV-RNA含量差异无统计学意义(t=1.20,P>0.05)。结论 性别和年龄与CHC 患者TLR 的表达有一定的关系,为临床合理有效治疗CHC 提供理论依据和新方法。
目的:觀察不同性彆和年齡慢性丙型肝炎(CHC)患者外週血單箇覈細胞(PBMC)中Toll樣受體3(TLR3)和 Toll樣受體7(TLR7)mRNA錶達,探討性彆和年齡在CHC患者外週血TLR3和TLR7基因錶達中的影響。方法選擇 CHC患者115例,健康人群113例,取抗凝全血,實時熒光定量PCR檢測TLR3和TLR7 mRNA錶達。結果 CHC患者TLR3和TLR7錶達量與健康人群相比,差異均有統計學意義(t=38.73,6.16,P<0.05)。CHC患者中非絕經期女性 TLR3和TLR7錶達量與絕經期女性和青年男性相比,差異均有統計學意義(t=61.210,6.464,P<0.05;t=24.166,26.266,P<0.05)。CHC患者中老年男性 TLR3和 TLR7錶達量與青年男性相比,差異均有統計學意義(t=86.349,19.583,P<0.05),而與絕經期女性相比,僅TLR3錶達量差異有統計學意義(t=122.941,P<0.05)。不同性彆和年齡 CHC患者的TLR3,TLR7基因錶達量與 HCV-RNA載量均無相關性(|r|<0.40,P>0.05),但是青年女性 CHC患者 HCV含量均比青年男性和老年女性低,差異有統計學意義(t=3.49,2.51,P<0.05),老年男性患者 HCV含量比青年男性低,差異有統計學意義(t=2.35,P<0.05),老年男性和女性 CHC患者 HCV-RNA含量差異無統計學意義(t=1.20,P>0.05)。結論 性彆和年齡與CHC 患者TLR 的錶達有一定的關繫,為臨床閤理有效治療CHC 提供理論依據和新方法。
목적:관찰불동성별화년령만성병형간염(CHC)환자외주혈단개핵세포(PBMC)중Toll양수체3(TLR3)화 Toll양수체7(TLR7)mRNA표체,탐토성별화년령재CHC환자외주혈TLR3화TLR7기인표체중적영향。방법선택 CHC환자115례,건강인군113례,취항응전혈,실시형광정량PCR검측TLR3화TLR7 mRNA표체。결과 CHC환자TLR3화TLR7표체량여건강인군상비,차이균유통계학의의(t=38.73,6.16,P<0.05)。CHC환자중비절경기녀성 TLR3화TLR7표체량여절경기녀성화청년남성상비,차이균유통계학의의(t=61.210,6.464,P<0.05;t=24.166,26.266,P<0.05)。CHC환자중노년남성 TLR3화 TLR7표체량여청년남성상비,차이균유통계학의의(t=86.349,19.583,P<0.05),이여절경기녀성상비,부TLR3표체량차이유통계학의의(t=122.941,P<0.05)。불동성별화년령 CHC환자적TLR3,TLR7기인표체량여 HCV-RNA재량균무상관성(|r|<0.40,P>0.05),단시청년녀성 CHC환자 HCV함량균비청년남성화노년녀성저,차이유통계학의의(t=3.49,2.51,P<0.05),노년남성환자 HCV함량비청년남성저,차이유통계학의의(t=2.35,P<0.05),노년남성화녀성 CHC환자 HCV-RNA함량차이무통계학의의(t=1.20,P>0.05)。결론 성별화년령여CHC 환자TLR 적표체유일정적관계,위림상합리유효치료CHC 제공이론의거화신방법。
Objective Investigating the expression of TLR3 and TLR7 mRNA in peripheral blood mononuclear cells (PBMC) of patients with chronic hepatitis C infection (CHC),to explore the effects of gender and age on Toll-like receptor expres-sion.Methods Peripheral blood was collected from 1 1 5 patients of chronic hepatitis C infection,1 1 3 healthy individuals.Ex-pression levels of TLR3 and TLR7 mRNA were detected by real-time quantitative PCR.Results The expression of TLR3 and TLR7 mRNA were significant difference between patients with CHC infection and healthy individuals (t=38.73,6.16, P<0.05),respectively.The expression of TLR3 and TLR7 mRNA were significant difference between premenopausal fe-males with CHC infection,postmenopausal females and young males of CHC infection (t=61.210,6.464,P<0.05;t=24.166,26.266,P<0.05),respectively.The expression of TLR3 and TLR7 mRNA were significant differences between old males and young males of chronic hepatitis CHC infection (t=86.349,19.583,P<0.05).The expression of TLR3 mRNA was significant differences between old males and postmenopausal females of CHC infection (t=122.941,P<0.05).There was no correlation between the expressions of TLR3 and TLR7 and HCV-RNA load of CHC patents of Gender and age dis-crepancies (|r|<0.40,P>0.05).The HCV-RNA load of premenopausal females was significant lower than young males and old females (t=3.49,2.51,P<0.05),the load of old males was lower than old females (t=2.35,P<0.05),however, there was no significant differences between old males and old females (t=1.20,P<0.05).Conclusion Gender and age dis-crepancies have a relationship with the expression of Toll-like receptors of patients with CHC infection,which may provide a theoretical basis and a new method for CHC.