CAJ | 학술논문

目的：探讨重度乳糜血标本对比色终点法、速率法以及透射比浊法三种常见类型生化检测方法所造成影响的差别。方法选取外观正常的混合血清标本20份，每份分成 A，B，C，D，E，F和 G 7个1 ml血清的实验组与1个对照组，实验组从A～G依次加入脂肪乳液10，20，40，80，160，320μl和640μl配制成浊度呈递增式的模拟乳糜血清标本。各血清均进行以葡萄糖（Glu）、尿酸（UA）、总胆红素（TBil）、天门冬氨酸氨基转移酶（AST）、γ-谷氨酰转肽酶（GGT）、乳酸脱氢酶（LDH）、超敏C反应蛋白（hs-CRP）、载脂蛋白 A1（apoA1）、β2微球蛋白（β2-MG）作为代表的比色终点法、速率法以及透射比浊法三种常见反应类型生化项目的检测，各实验组换算后结果与对照组分别进行配对t检验。结果透射比浊法中三个生化项目所有实验组与对照组差异均有统计学意义（t＝－2.842～29.465，P 值均＜0.05）。比色终点法中除了 Glu实验组 A组与对照组差异无统计学意义（t＝1.875，P＝0.068＞0.05）外，其它实验组与对照组差异均有统计学意义（t＝－34.942～13.884，P值均＜0.05）。速率法中除了 GGT实验组 B，C组与对照组差异有统计学意义（t＝2.234和5.006，P值均＜0.05）外，其它可检测的实验组与对照组差异均无统计学意义（t＝－1.532～1.619，P值均＞0.05）。结论三种常见类型生化检测方法中乳糜血标本对比色终点法和透射比浊法影响较大，对速率法影响相对较小。
목적：탐토중도유미혈표본대비색종점법、속솔법이급투사비탁법삼충상견류형생화검측방법소조성영향적차별。방법선취외관정상적혼합혈청표본20빈，매빈분성 A，B，C，D，E，F화 G 7개1 ml혈청적실험조여1개대조조，실험조종A～G의차가입지방유액10，20，40，80，160，320μl화640μl배제성탁도정체증식적모의유미혈청표본。각혈청균진행이포도당（Glu）、뇨산（UA）、총담홍소（TBil）、천문동안산안기전이매（AST）、γ-곡안선전태매（GGT）、유산탈경매（LDH）、초민C반응단백（hs-CRP）、재지단백 A1（apoA1）、β2미구단백（β2-MG）작위대표적비색종점법、속솔법이급투사비탁법삼충상견반응류형생화항목적검측，각실험조환산후결과여대조조분별진행배대t검험。결과투사비탁법중삼개생화항목소유실험조여대조조차이균유통계학의의（t＝－2.842～29.465，P 치균＜0.05）。비색종점법중제료 Glu실험조 A조여대조조차이무통계학의의（t＝1.875，P＝0.068＞0.05）외，기타실험조여대조조차이균유통계학의의（t＝－34.942～13.884，P치균＜0.05）。속솔법중제료 GGT실험조 B，C조여대조조차이유통계학의의（t＝2.234화5.006，P치균＜0.05）외，기타가검측적실험조여대조조차이균무통계학의의（t＝－1.532～1.619，P치균＞0.05）。결론삼충상견류형생화검측방법중유미혈표본대비색종점법화투사비탁법영향교대，대속솔법영향상대교소。
Objective Aim to explore how severe chyle blood serum effects on the End-point method of colorimetry,velocity method,immunological transmission turbidimetry(TIA)three types of common biochemical tests and their different influ-ences.Methods Collected 20 normal appearance serums,divided each pooled serum into A,B,C,D,E,F and G 7 experimen-tal groups and one control group.Each group contented for 1ml serum.Added intralipid 10,20,40,80,160μl and 320μl to the experimental groups from A to G in turn to prepare into different concentrations of simulate chylous samples.Tested each group by the End-point method of colorimetry,velocity method and TIA which represented by Glucose (Glu),uric acid (UA),Total bilirubin (TBil),aspartate aminotransferase (AST),gamma-glutamyl transpeptidase (GGT),lactate dehydro-genase (LDH),hypersensitive C reactive protein (hs-CRP),Apolipoprotein A1 (apoA1 )andβ2-microglobulin (β2-MG). Used pair T test for both experimental group and their control group.Results In terms of TIA,there were statistical differ-ences between all the experimental groups and their control groups in the three projects (t=-2.842~29.465,P<0.05).In terms of the End-point method of colorimetry,there were statistical differences between all the experimental groups and their control groups (t=-34.942~13.884,P<0.05)except for A group in Glu experiment (t=1.875,P=0.068>0.05).In terms of velocity method,there were no statistical differences between all the experimental groups and their control groups (t=-1.532~1.619,P>0.05)except for the B and C groups in GGT experiment (t=2.234&5.006,P<0.05).Conclusion Severe chyle blood serum had significant influence on the End-point method of colorimetry and TIA,as well as less influ-ence on velocity method.