CAJ | 학술논문

目的：探讨胃癌细胞中人类相关转录因子3（RUNX3）对miRNA‐106a（miR‐106a）表达的影响以及与多药耐药的关系。方法采用免疫印迹法及细胞凋亡检测来观察miR‐106a在两个人类胃癌细胞多药耐药细胞系上的表达情况。运用免疫印迹及多聚酶链式反应检测miR‐106a的表达来观察癌细胞对抗癌药物的敏感性。通过荧光素酶活性测定观察miR‐106a与RU NX3的关系。结果 miR‐106a在多药耐药胃癌细胞中表达增加，并抑制胃癌细胞对抗癌药物的敏感性；通过作用于RUNX3调节多药耐药。结论通过作用于RUNX3基因，miR‐106a诱导胃癌多药耐药性。
목적：탐토위암세포중인류상관전록인자3（RUNX3）대miRNA‐106a（miR‐106a）표체적영향이급여다약내약적관계。방법채용면역인적법급세포조망검측래관찰miR‐106a재량개인류위암세포다약내약세포계상적표체정황。운용면역인적급다취매련식반응검측miR‐106a적표체래관찰암세포대항암약물적민감성。통과형광소매활성측정관찰miR‐106a여RU NX3적관계。결과 miR‐106a재다약내약위암세포중표체증가，병억제위암세포대항암약물적민감성；통과작용우RUNX3조절다약내약。결론통과작용우RUNX3기인，miR‐106a유도위암다약내약성。
Objective To explore the effect of miRNA‐106a(miR‐106a) expression on multidrug resistance(MDR)of gastric cancer(GC)cells and the involvement of runt‐related transcription factor 3 RUNX3.Methods The expression of miR‐106a was detected in two human gastric adenocarcinoma cell lines with MDR by immunoblotting and apoptosis assay. The sensitivity of GC cells to anticancer drugs was observed by detecting the expression of miR‐106a by using immunoblotting and PCR ,and the relationship between miR‐106a and RUNX3 was determined by luciferase activity assay.Results miR‐106a was significantly in‐creased in GC cells with MDR ,and it suppressed the sensitivity of GC cells to anticancer drugs. It could modulate MDR by tar‐geting RUNX3.Conclusion miR‐106a can induce the MDR by targeting RUNX3 in GC.