华中科技大学学报（医学版）
華中科技大學學報（醫學版）
화중과기대학학보（의학판）
ACTA UNIVERSITATIS MEDICINAE TONGJI
2015年
1期
37-41
,共5页
黄丙清%邱香%王林枝%朱腾世%郭琰芳%郝丽萍
黃丙清%邱香%王林枝%硃騰世%郭琰芳%郝麗萍
황병청%구향%왕림지%주등세%곽염방%학려평
酒精性肝损伤%白藜芦醇%乙醇脱氢酶%乙醛脱氢酶2
酒精性肝損傷%白藜蘆醇%乙醇脫氫酶%乙醛脫氫酶2
주정성간손상%백려호순%을순탈경매%을철탈경매2
alcoholic liver injury%resveratrol%ADH%ALDH2
目的：探讨酒精代谢酶乙醇脱氢酶（ADH）和乙醛脱氢酶2（ALDH2）在白藜芦醇（resveratrol ，Res）改善大鼠慢性酒精性肝损伤中的机制。方法采用含酒精的Lieber‐DeCarli液体饲料喂养大鼠19周，建立慢性酒精性肝损伤模型。42只SD大鼠随机分成正常对照组，低、中、高剂量酒精组，Res对照组，高剂量酒精＋ Res组。采用苏木精‐伊红（HE）和油红O染色，观察肝组织形态学改变；采用试剂盒测定肝组织中ADH、ALDH2的活性；Western blot检测肝组织中ADH与ALDH2的蛋白表达。结果各剂量酒精组大鼠肝组织脂肪变性和炎症浸润明显，体重和能量摄入量与正常对照组相比下降，差异具有统计学意义（P＜0.05或 P＜0.01）；且肝组织ADH活性与蛋白表达略有升高，而肝组织ALDH2活性与蛋白表达显著下降。Res干预后大鼠肝组织脂肪变性和炎症浸润均较高剂量酒精组显著减轻，体重与能量摄入量增加，显著提升了肝组织中ALDH2的活性与蛋白表达（P＜0.05）。结论 Res可能通过对大鼠肝脏ALDH2的调节来改善慢性酒精摄入引起的肝脏损伤。
目的：探討酒精代謝酶乙醇脫氫酶（ADH）和乙醛脫氫酶2（ALDH2）在白藜蘆醇（resveratrol ，Res）改善大鼠慢性酒精性肝損傷中的機製。方法採用含酒精的Lieber‐DeCarli液體飼料餵養大鼠19週，建立慢性酒精性肝損傷模型。42隻SD大鼠隨機分成正常對照組，低、中、高劑量酒精組，Res對照組，高劑量酒精＋ Res組。採用囌木精‐伊紅（HE）和油紅O染色，觀察肝組織形態學改變；採用試劑盒測定肝組織中ADH、ALDH2的活性；Western blot檢測肝組織中ADH與ALDH2的蛋白錶達。結果各劑量酒精組大鼠肝組織脂肪變性和炎癥浸潤明顯，體重和能量攝入量與正常對照組相比下降，差異具有統計學意義（P＜0.05或 P＜0.01）；且肝組織ADH活性與蛋白錶達略有升高，而肝組織ALDH2活性與蛋白錶達顯著下降。Res榦預後大鼠肝組織脂肪變性和炎癥浸潤均較高劑量酒精組顯著減輕，體重與能量攝入量增加，顯著提升瞭肝組織中ALDH2的活性與蛋白錶達（P＜0.05）。結論 Res可能通過對大鼠肝髒ALDH2的調節來改善慢性酒精攝入引起的肝髒損傷。
목적：탐토주정대사매을순탈경매（ADH）화을철탈경매2（ALDH2）재백려호순（resveratrol ，Res）개선대서만성주정성간손상중적궤제。방법채용함주정적Lieber‐DeCarli액체사료위양대서19주，건립만성주정성간손상모형。42지SD대서수궤분성정상대조조，저、중、고제량주정조，Res대조조，고제량주정＋ Res조。채용소목정‐이홍（HE）화유홍O염색，관찰간조직형태학개변；채용시제합측정간조직중ADH、ALDH2적활성；Western blot검측간조직중ADH여ALDH2적단백표체。결과각제량주정조대서간조직지방변성화염증침윤명현，체중화능량섭입량여정상대조조상비하강，차이구유통계학의의（P＜0.05혹 P＜0.01）；차간조직ADH활성여단백표체략유승고，이간조직ALDH2활성여단백표체현저하강。Res간예후대서간조직지방변성화염증침윤균교고제량주정조현저감경，체중여능량섭입량증가，현저제승료간조직중ALDH2적활성여단백표체（P＜0.05）。결론 Res가능통과대대서간장ALDH2적조절래개선만성주정섭입인기적간장손상。
Objective To evaluate the protective effects of resveratrol (Res)on chronic alcoholic liver injury in rats and fur‐ther explore the underlying mechanism involving hepatic ethanol metabolizing enzymes (ADH and ALDH2).Methods Alcoholic liver injury models of rat were established by feeding Lieber‐DeCarli liquid ethanol diet for 19 weeks. Forty‐two SD rats were randomly divided into normal control group ,low‐,middle‐and high‐dose ethanol groups ,Res control group and high‐dose etha‐nol +Res group. The morphological change of hepatic tissues was observed by hematoxylin‐eosin(HE)and oil red O staining ;the activity of hepatic ADH and ALDH2 was determined by using appropriate kits ;and the protein expressions of hepatic ADH and ALDH2 were detected by Western blot.Results There were serious hepatic steatosis and inflammation in ethanol groups.Body weight and food intake were significantly decreased in the ethanol groups compared with the normal control group. There was moderate increase in the ADH activity and expression and significant decrease in ALDH 2 activity and expres‐sion in ethanol groups.Res could ameliorate ethanol‐induced hepatic steatosis and inflammation as well as increase body weight and energy intake. Hepatic ALDH2 activity and protein expression were significantly increased by Res treatment (P<0.05) . Conclusion Res could improve chronic ethanol‐induced liver injury by regulating hepatic ALDH 2 expression.
