CAJ | 학술논문

目的：比较分析髓源性抑制细胞（myeloid‐derived suppressor cells ，MDSCs）在健康人、宫颈上皮内瘤变患者、宫颈癌患者及其术后外周血中的比例，初步探讨其临床意义。方法采用流式细胞术检测36例宫颈癌患者、52例宫颈上皮内瘤变患者及20例健康志愿者外周血中MDSCs、调节性T细胞（Treg）的比例。检测20例宫颈癌患者术前术后外周血中MDSCs、Treg的比例及MDSCs产生的精氨酸酶和一氧化氮（NO）的变化情况。结果宫颈癌组外周血中MD‐SCs、Treg的比例最高，分别为[（3.85±0.85）％，（7.82±1.04）％]，其次是宫颈上皮内瘤变组[（1.52±0.53）％，（3.32±0.48）％]，健康对照组最低[（0.61±0.17）％，（2.02±0.26）％]，各组间差异均有统计学意义（均 P＜0.05）；手术后，患者外周血中MDSCs、Treg的比例明显下降，MDSCs产生的精氨酸酶和NO下降，与术前比较差异均有统计学意义（均 P＜0.05）。结论外周血MDSCs比例的升高可能与肿瘤免疫功能低下及宫颈癌的发生发展密切相关。
목적：비교분석수원성억제세포（myeloid‐derived suppressor cells ，MDSCs）재건강인、궁경상피내류변환자、궁경암환자급기술후외주혈중적비례，초보탐토기림상의의。방법채용류식세포술검측36례궁경암환자、52례궁경상피내류변환자급20례건강지원자외주혈중MDSCs、조절성T세포（Treg）적비례。검측20례궁경암환자술전술후외주혈중MDSCs、Treg적비례급MDSCs산생적정안산매화일양화담（NO）적변화정황。결과궁경암조외주혈중MD‐SCs、Treg적비례최고，분별위[（3.85±0.85）％，（7.82±1.04）％]，기차시궁경상피내류변조[（1.52±0.53）％，（3.32±0.48）％]，건강대조조최저[（0.61±0.17）％，（2.02±0.26）％]，각조간차이균유통계학의의（균 P＜0.05）；수술후，환자외주혈중MDSCs、Treg적비례명현하강，MDSCs산생적정안산매화NO하강，여술전비교차이균유통계학의의（균 P＜0.05）。결론외주혈MDSCs비례적승고가능여종류면역공능저하급궁경암적발생발전밀절상관。
Objective To investigate the proportion of circulating myeloid‐derived suppressor cells(MDSCs)in the peripher‐al blood of healthy volunteers ,patients with cervical intraepithelial neoplasia ,patients with cervical cancer before and after oper‐ation.Methods Flow cytometry was used to evaluate the proportion of MDSCs and Treg in the peripheral blood of 36 patients with cervical cancer ,52 patients with intraepithelial neoplasia and 20 healthy people. The proportions of MDSCs and Treg were evaluated in 20 patients with cervical cancer before and after operation as well as the changes of argininase activity and nitric ox‐ide(NO) production.Results The proportions of circulating MDSCs and Tregs were(3.85 ± 0.85)% and (7.82 ± 1.04)% ,re‐spectively ,in patients with cervical cancer ,which was highest among the subjects. Those in patients with cervical intraepithelial neoplasia were(1.52 ± 0.53)% and (3.32 ± 0.48)% ,respectively ,significantly lower than those in cervical cancer patients but much higher than in healthy volunteers[(0.61 ± 0.17)% ,(2.02 ± 0.26)% ].The differences between groups were statistically significant(P<0.05). The proportions of MDSCs and Treg were conspicuously decreased ,and the argininase activity and nitric oxide production were also reduced in patients with cervical cancer after the operation when compared with those before the op‐eration.The differences were statistically significant(P<0.05).Conclusion The increased proportion of MDSCs in the periph‐eral blood of patients with cervical cancer may closely correlate with the poor antitumor immune function of patients and the de‐velopment of cervical cancer.