CAJ | 학술논문

目的：制备新型分子影像探针标记间充质干细胞，以实现细胞荧光成像。方法应用1‐碘十二烷修饰聚乙烯亚胺，构建两亲性大分子。利用大分子自组装性能，包裹疏水性核壳结构CdSe/CdS量子点构建纳米探针。结果该探针在630 nm处呈现出较强的荧光信号，能够促使间充质干细胞高效内吞。体内成像结果显示，与空白对照细胞相比，被标记细胞呈现出显著的光学信号，空白组信号值为1.47 e8[p/s/cm2/sr]/[μW/cm2]，实验组信号值为2.78 e8[p/s/cm2/sr]/[μW/cm2]。结论该纳米探针具有良好光学成像性能，可实现细胞标记。
목적：제비신형분자영상탐침표기간충질간세포，이실현세포형광성상。방법응용1‐전십이완수식취을희아알，구건량친성대분자。이용대분자자조장성능，포과소수성핵각결구CdSe/CdS양자점구건납미탐침。결과해탐침재630 nm처정현출교강적형광신호，능구촉사간충질간세포고효내탄。체내성상결과현시，여공백대조세포상비，피표기세포정현출현저적광학신호，공백조신호치위1.47 e8[p/s/cm2/sr]/[μW/cm2]，실험조신호치위2.78 e8[p/s/cm2/sr]/[μW/cm2]。결론해납미탐침구유량호광학성상성능，가실현세포표기。
Objective Stem cell therapy has been extensively used in clinical practice. However ,the methods available are unable to non‐invasively detect the distribution of cells in vivo. This study was aimed to establish a novel molecular imaging probe for stem cell labeling ,which would monitor cells via fluorescence imaging. Methods 1‐iodododecane was used to modify polyethyleneimine (PEI) to form amphiphilic macromolecules. These macromolecules encapsulated the hydrophobic quantum dots by self‐assembly to form imaging probes. Results Strong fluorescence signals of the probe occurred at 630 nm ,and the probe could be effectively endocyted by mesenchyme stem cells. In vivo imaging showed that significant optic signals occurred in probe‐labeled mesenchyme stem cells compared with the control cells (blank group:1.47e8 [p/s/cm2/sr]/[μW/cm2 ];test group:2.78e8 [p/s/cm2/sr]/[μW/cm2 ]). Conclusion The nanoprobes have good optic imaging effects ,and can be used to la‐bel cells.