CAJ | 학술논문

目的：观察小鼠体内传代与氟康唑体外诱导对阿萨希毛孢子菌(T.asahii)形态及药物敏感性的影响。方法将4株T.asahii在小鼠体内5次传代后，观察传代前后菌株形态和氟康唑最小抑菌浓度(minimal inhibitory concentration，MIC)；选择临床株CBS 2479和环境株CBS 8904，在含不同浓度氟康唑的固体培养基中体外诱导，观察诱导前后菌株形态和MIC值的变化。结果体内传代后所有菌株的MIC值和菌落形态较传代前均有较明显变化，其中临床株的MIC值较传代前增长8倍，而环境株的光镜下形态变化较明显，从以孢子状态为主变为孢子和菌丝的混合状态。体外诱导后，临床株和环境株的MIC值均＞256μg/ml，以临床株的MIC值变化幅度最大，其光镜下细胞形态也从诱导前的菌丝状态为主转化为孢子状态为主。结论T. asahii体内传代及体外诱导前后形态及药物敏感性均发生变化。体内传代后环境株形态有向临床株转变的趋势，而体外诱导后临床株逐渐向环境株转变。临床株标准株两种情况下氟康唑MIC值增高均以临床株更为显著，体外诱导较体内传代氟康唑MIC值增长更高、更快。
목적：관찰소서체내전대여불강서체외유도대아살희모포자균(T.asahii)형태급약물민감성적영향。방법장4주T.asahii재소서체내5차전대후，관찰전대전후균주형태화불강서최소억균농도(minimal inhibitory concentration，MIC)；선택림상주CBS 2479화배경주CBS 8904，재함불동농도불강서적고체배양기중체외유도，관찰유도전후균주형태화MIC치적변화。결과체내전대후소유균주적MIC치화균락형태교전대전균유교명현변화，기중림상주적MIC치교전대전증장8배，이배경주적광경하형태변화교명현，종이포자상태위주변위포자화균사적혼합상태。체외유도후，림상주화배경주적MIC치균＞256μg/ml，이림상주적MIC치변화폭도최대，기광경하세포형태야종유도전적균사상태위주전화위포자상태위주。결론T. asahii체내전대급체외유도전후형태급약물민감성균발생변화。체내전대후배경주형태유향림상주전변적추세，이체외유도후림상주축점향배경주전변。림상주표준주량충정황하불강서MIC치증고균이림상주경위현저，체외유도교체내전대불강서MIC치증장경고、경쾌。
ObjectiveTo investigate the change of morphology and antifungal drug susceptibility inT.asahii by murine passage and lfuconazole in vitro induction. MethodsFourT.asahii strains were ifve passaged through murine hosts; the shape of colony and cell type were observed, and the minimal inhibitory concentration(MIC) to lfuconazole were measured for the prepassage and postpassage isolates. Furthermore, twoT.asahii strains, including a clinical isolate CBS2479 and an environmental isolate CBS 8904, were respectively and serially subcultured in solid medium containing growing concentrations of lfuconazole. Changes in morphology and MIC value of the induced isolates were observed.ResultsIn all the passaged isolates in vivo and induced isolates in vitro, morphologies and drug susceptibilities to lfuconazole were signiifcantly different from the original isolates, where the lfuconazole MIC value of clinical passaged isolates was increased 8 times relates to prepassage MIC, and cell type of environmental isolates, which the morphological characteristics observed with microscopes, changed from dominated by spores to containing spores and hyphae. After in-vitro induction, high level of lfuconazole resistance (MIC>256 μg/ml) developed in both the clinical isolates and the environmental isolates, and the latter changed their MIC value more signiifcantly, meanwhile from dominated by hyphae to spores of cell types. ConclusionMorphologies and antifungal susceptibilities to lfuconazole inT.asahii were changed by in-vivo passage and in-vitro induction. The morphologies of passaged environmental isolates in vivo have trends to the clinical isolate, which is contrary with in-vitro induction. Both in-vivo passage and in-vitro induction, the increased range of lfuconazole MIC value in the clinical isolates is signiifcantly greater than environmental isolates. The lfuconazole in-vitro induction increased higher and faster level of MIC value then in-vivo passage.