CAJ | 학술논문

采用稀释平板法和Biolog-ECO微平板技术，以剌槐（Robinia pseudoacacia）天然次生林为研究对象，研究湖南省衡阳市紫色土丘陵坡地表层（0~10 cm）土壤微生物群落的季节变化特征。结果表明：1）夏季，土壤微生物总数，细菌数量、真菌数量和放线菌数量最高，分别为16.34×106、16.09×106、10.43×104和14.64×104 cfu·g-1干土，春季次之：11.61×106、11.45×106、5.00×104和10.65×104 cfu·g-1干土，秋季最低：5.87×106、5.78×106、4.67×104和4.08×104 cfu·g-1干土，春、夏和秋3季的差异达显著水平（P<0.05）；2）在培养168 h时，土壤微生物C源平均颜色变化率（Average well color development， AWCD）以夏季最高（1.20），春季次之（0.88），秋季最低（0.83）；3）土壤微生物功能多样性表现为夏季明显高于春、秋2季（P<0.05），夏季的Patrick丰富度指数（R）（28）、Shannon-Wiener指数（H）（3.22）、Simpson指数（D）（0.96）和McIntosh指数（U）（8.20）显著高于春、秋2季（P<0.05），而春、秋2季的R（23，24）、H（3.06，3.08）、D（0.95，0.95）和U（5.90，5.91）无显著差异（P>0.05）；4）氨基酸类、聚合物类和羧酸C源类是衡阳紫色土丘陵坡地土壤微生物偏好且利用率较高的C源类型；5）主成分分析表明，土壤微生物群落的C源利用可分为2类，一类在夏季，另一类在春、秋2季，其得分系数的分布范围分别为（2.59~6.00，2.43~5.09）和（-7.65~-1.90，-6.38~-3.43）。研究结果为科学评价湖南省衡阳市紫色土丘陵坡地土壤生境质量退化和恢复过程中微生物特征的变化提供了本底值参考。
채용희석평판법화Biolog-ECO미평판기술，이랄괴（Robinia pseudoacacia）천연차생림위연구대상，연구호남성형양시자색토구릉파지표층（0~10 cm）토양미생물군락적계절변화특정。결과표명：1）하계，토양미생물총수，세균수량、진균수량화방선균수량최고，분별위16.34×106、16.09×106、10.43×104화14.64×104 cfu·g-1간토，춘계차지：11.61×106、11.45×106、5.00×104화10.65×104 cfu·g-1간토，추계최저：5.87×106、5.78×106、4.67×104화4.08×104 cfu·g-1간토，춘、하화추3계적차이체현저수평（P<0.05）；2）재배양168 h시，토양미생물C원평균안색변화솔（Average well color development， AWCD）이하계최고（1.20），춘계차지（0.88），추계최저（0.83）；3）토양미생물공능다양성표현위하계명현고우춘、추2계（P<0.05），하계적Patrick봉부도지수（R）（28）、Shannon-Wiener지수（H）（3.22）、Simpson지수（D）（0.96）화McIntosh지수（U）（8.20）현저고우춘、추2계（P<0.05），이춘、추2계적R（23，24）、H（3.06，3.08）、D（0.95，0.95）화U（5.90，5.91）무현저차이（P>0.05）；4）안기산류、취합물류화최산C원류시형양자색토구릉파지토양미생물편호차이용솔교고적C원류형；5）주성분분석표명，토양미생물군락적C원이용가분위2류，일류재하계，령일류재춘、추2계，기득분계수적분포범위분별위（2.59~6.00，2.43~5.09）화（-7.65~-1.90，-6.38~-3.43）。연구결과위과학평개호남성형양시자색토구릉파지토양생경질량퇴화화회복과정중미생물특정적변화제공료본저치삼고。
Seasonal variations of soil microbial communities in surface soil(0~10 cm) of Robinia pseudoacacia natural secondary forest on sloping-land with purple soils in Hengyang city of Hunan province, south-central China was studied by using the method of dilution-plate method and Biolog ECO-microplate culture. The results showed that:(1)The quantity of soil microorganism, bacteria, fungi and actiomycetes was the maximum in summer, they was 16.34×106, 16.09×106, 10.43×104 and 14.64×104 cfu·g-1 dry soil, respectively, fewer in spring, 11.61×106, 11.45×106, 5.00×104 and 10.65×104 cfu·g-1 dry soil, respectively, the minimum in autumn, 5.87×106, 5.78×106, 4.67×104 and 4.08×104 cfu·g-1 dry soil, respectively, there existed significantly difference among the microbe quantity of three seasons(P<0.05); (2)The peak value of AWCD(Average well color development) was found in summer(1.20), followed by spring(0.88) and autumn(0.83) in consequence;(3)The microbial functional diversity was significantly higher in summer than in spring and autumn(P<0.05), and showed no significant difference in spring and autumn(P>0.05), for summer, Patrick richness index(R)(28), Shannon-Wiener index(H)(3.22), Simpson index(D)(0.96) and McIntosh index(U)(8.20), for spring and autumn, R(23, 24), H(3.06, 3.08), D(0.95, 0.95) and U(5.90, 5.91);(4)Amino acids, ployers and carboxylic acids were the main C sources utilized by the microbial communities;(4)PCA(Principal component analysis) showed the C sources by soil microbes were divided into two categories, one in summer, another, spring and autumn, the range of score coefficient were (2.59_6.00, 2.43_5.09) and (-7.65_-1.90,-6.38_-3.43), respectively. Above all, this result would provide more important primitive properties of microbial features in the degradation and restoration process of the quality of the soil habitat scientifically on the sloping-land with purple soils in Hengyang of Hunan Province, South-central China.