CAJ | 학술논문

酞酸酯（PAEs, phthalate acid esters）是目前最普遍的有机污染物之一，一定剂量的酞酸酯会产生生殖和发育毒性等健康危害。以明亮发光杆菌（Photobacterium phosphoreum T3）为受试生物，评价了甲醇和3种PAEs [dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP)]对其剂量—效应关系，以期为酞酸酯暴露风险评价提供一定理论依据。结果表明，采用Logit和Weibull非线性函数（全浓度区间）分别对甲醇和3种PAEs的浓度—效应曲线进行拟合，效果均优于线性函数（局部浓度区间）拟合。其中，甲醇的线性与Logit函数拟合r2分别为0.9592和0.9863，两者EC50分别为1.185和1.044 mol·L-1，结果相近。数据结果表明，采用明亮发光杆菌测试PAEs急性毒性时，以甲醇为助溶剂（浓度<0.247 mol·L-1，毒性效应可忽略）是可行的。在一定浓度范围内，首次以甲醇为助溶剂，以DMP?DEP和DBP 3种PAEs对明亮发光杆菌进行毒性测试。线性拟合DMP?DEP和DBP对明亮发光杆菌的浓度-效应曲线，拟合r2分别为0.9262、0.9214、0.9339，其EC50分别为2.64E-04、2.79E-04和1.14E-05 mol·L-1。与此同时，3组PAEs毒性数据均能以Weibull函数进行非线性拟合， r2分别为0.9749?0.9742和0.9648，效果良好，其EC50分别为1.49E-04、2.72E-04和9.00E-06 mol·L-1。两种拟合结果表明， Weibull 函数非线性拟合效果更优，半致死浓度 EC50结果相近。由上述结果可知，以甲醇为助溶剂时，DMP?DEP 和 DBP分别在6.14E-9~6.14E-3、5.04E-5~2.52E-3和3.76E-9~3.76E-5 mol·L-1浓度范围内，3种PAEs对明亮发光杆菌的急性毒性强弱为DBP>DMP>DEP。在上述浓度范围内，DMP、DEP和DBP对明亮发光杆菌的抑制率分别为96.87%、95.24%和88.35%。
태산지（PAEs, phthalate acid esters）시목전최보편적유궤오염물지일，일정제량적태산지회산생생식화발육독성등건강위해。이명량발광간균（Photobacterium phosphoreum T3）위수시생물，평개료갑순화3충PAEs [dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP)]대기제량—효응관계，이기위태산지폭로풍험평개제공일정이론의거。결과표명，채용Logit화Weibull비선성함수（전농도구간）분별대갑순화3충PAEs적농도—효응곡선진행의합，효과균우우선성함수（국부농도구간）의합。기중，갑순적선성여Logit함수의합r2분별위0.9592화0.9863，량자EC50분별위1.185화1.044 mol·L-1，결과상근。수거결과표명，채용명량발광간균측시PAEs급성독성시，이갑순위조용제（농도<0.247 mol·L-1，독성효응가홀략）시가행적。재일정농도범위내，수차이갑순위조용제，이DMP?DEP화DBP 3충PAEs대명량발광간균진행독성측시。선성의합DMP?DEP화DBP대명량발광간균적농도-효응곡선，의합r2분별위0.9262、0.9214、0.9339，기EC50분별위2.64E-04、2.79E-04화1.14E-05 mol·L-1。여차동시，3조PAEs독성수거균능이Weibull함수진행비선성의합， r2분별위0.9749?0.9742화0.9648，효과량호，기EC50분별위1.49E-04、2.72E-04화9.00E-06 mol·L-1。량충의합결과표명， Weibull 함수비선성의합효과경우，반치사농도 EC50결과상근。유상술결과가지，이갑순위조용제시，DMP?DEP 화 DBP분별재6.14E-9~6.14E-3、5.04E-5~2.52E-3화3.76E-9~3.76E-5 mol·L-1농도범위내，3충PAEs대명량발광간균적급성독성강약위DBP>DMP>DEP。재상술농도범위내，DMP、DEP화DBP대명량발광간균적억제솔분별위96.87%、95.24%화88.35%。
Phthalate acid esters (PAEs) are one of the most common organic pollutants currently. A dose of PAEs would bring about health hazards such as reproductive and developmental toxicity. To provide some academic evidence of exposure and risk assessment to PAEs, the dose-response of methanol and the three PAEs (DMP, DEP and DBP) to Photobacterium phosphereum T3 was evaluated. The results showed that, the dose-response of methanol and the three PAEs could be separately better non-linear fitted by Logit and Weibull equations (in full concentration range) compared to linear fitting (part of the concentrations). For methanol, the correlation coefficients R2 of linear fitting and non-linear fitting were 0.959 2 and 0.986 3 separately. And their EC50 calculated by both ways were similar with results of 1.185 and 1.044 mol·L-1. The data results showed that it was feasible for methanol (with concentration of<0.247 mol·L-1) to act as co-solvents for the three PAEs (DMP, DEP and DBP) in acute toxicity test. The toxicity assessment of the three PAEs to Photobacterium phosphoreum T3 was performed in certain doses. Linear fitting was executed for the dose-response curves of DMP, DEP and DBP. Their EC50 values were 2.64E-04, 2.79E-04 and 1.14E-05 mol·L-1, while the three correlation coefficients r2 were 0.926 2, 0.921 4 and 0.933 9, respectively. In the meantime all the data could be well non-linear fitted by Weibull equation (in full concentration range). Their correlation coefficients R2 were 0.974 9, 0.974 2 and 0.964 8 respectively. The results were satisfied and their EC50 values were 1.49E-04, 2.72E-04 and 9.00E-06 mol·L-1. The both fitted data expressed that the Weibull non-linear was more fitted and their EC50 values were similar. By the results, for DMP, DEP and DBP with respective concentration ranges of 6.14E-9~6.14E-3, 5.04E-5~2.52E-3 and 3.76E-9~3.76E-5 mol·L-1, the acute toxicity to Photobacterium phosphoreum T3 was DBP>DMP>DEP. In the concentration ranges, the inhibiton ratios for DMP, DEP and DBP were 96.87%, 95.24%and 88.35%respectively.