CAJ | 학술논문

为了探究并优化菌剂应用于微污染水源水体修复的机制和条件，主要针对水库沉积物内筛选出的贫营养好氧反硝化菌进行了菌种鉴定及脱氮特性研究，考察菌株在不同环境条件下的脱氮效果，明确了该菌株的最适宜生长条件，并基于水库水体中贫营养条件对菌株进行水源水库原水的驯化培养试验研究，以期实现该菌株对微污染水源水库原水中氮源污染物的脱除，为原位投菌技术实际工程应用提供理论依据。从微污染水源水库沉积物中驯化筛分出一株高效异养硝化-好氧反硝化菌A14，通过扫描电镜观察、生理生化特征、16S rRNA基因测序和Biolog GenⅢ鉴定，确定该菌株为革兰氏阴性短杆菌，鉴定为皮特不动杆菌（Acinetobacter pittii）。在好氧条件下，菌株细胞内表达反硝化功能基因napA，以NO3-为唯一氮源进行反硝化作用时，36 h时NO3-去除率为78.89%。以NH4+为唯一氮源时，48 h NH4+去除率为95.25%，TN去除率达80.42%，TOC去除率达98.30%，表明该菌株具有异养硝化-好氧反硝化特性。在改变环境条件过程中，该菌株在以乙酸钠为碳源，温度为30℃，C/N为12，pH为7，接种量为10%时，NO3-去除率最高为86.62%，并且在10℃下脱氮率达到40.18%。在水源水库原水脱氮实验中，接种处理TN去除率为50.95%，NO3-去除率为80.25%。结果表明，菌株A14在微污染水源水体菌剂脱氮修复中具有良好的应用潜力。
위료탐구병우화균제응용우미오염수원수체수복적궤제화조건，주요침대수고침적물내사선출적빈영양호양반초화균진행료균충감정급탈담특성연구，고찰균주재불동배경조건하적탈담효과，명학료해균주적최괄의생장조건，병기우수고수체중빈영양조건대균주진행수원수고원수적순화배양시험연구，이기실현해균주대미오염수원수고원수중담원오염물적탈제，위원위투균기술실제공정응용제공이론의거。종미오염수원수고침적물중순화사분출일주고효이양초화-호양반초화균A14，통과소묘전경관찰、생리생화특정、16S rRNA기인측서화Biolog GenⅢ감정，학정해균주위혁란씨음성단간균，감정위피특불동간균（Acinetobacter pittii）。재호양조건하，균주세포내표체반초화공능기인napA，이NO3-위유일담원진행반초화작용시，36 h시NO3-거제솔위78.89%。이NH4+위유일담원시，48 h NH4+거제솔위95.25%，TN거제솔체80.42%，TOC거제솔체98.30%，표명해균주구유이양초화-호양반초화특성。재개변배경조건과정중，해균주재이을산납위탄원，온도위30℃，C/N위12，pH위7，접충량위10%시，NO3-거제솔최고위86.62%，병차재10℃하탈담솔체도40.18%。재수원수고원수탈담실험중，접충처리TN거제솔위50.95%，NO3-거제솔위80.25%。결과표명，균주A14재미오염수원수체균제탈담수복중구유량호적응용잠력。
To investigate the feasibility that the strain can used to treat micro-polluted water, the research is mainly about the identification and the denitrification characteristics of the aerobic-denitrification strain that was isolated from sediment in water reservoir and the effects of nitrogen removal under different environmental factors. The optimal conditions of the strain can be determined. Through the method of cultivation and acclimatization to make the strain adapt to the oligotrophic condition and make effects on nitrogen removal, which all prove a theoretical basis for the practical application of onsite biological treatment in the future. A high efficient heterotrophic nitrification-denitrification aerobic bacteria (A14) was isolated from sediment in water reservoir. Based on its microscopic observation, biochemical/morphological characteristics, its 16S rRNA sequence homologic analysis and Biolog GenⅢ identification, this strain was a gram-negative bacillus and was identified as Acinetobacter pittii. The efficient nitrogen removal in 36 hours can be 78.89%when nitrogen as the nitrogen source and the strain’s partial napA functional gene was cloned. When ammonia as the nitrogen source, TN removal rate can be 80.42%, ammonia removal rate can be 95.25%, TOC removal rate can be 98.3%. The highest nitrite removal rate reaches 86.62% when carbon resource is NaAc, temperature=30 ℃, C/N=12, pH=7, inoculating ratio is 10%. In addition, the nitrite removal rate can reach 40.18%under 10 ℃. When the strain is cultivated and acclimated in reservoir raw water, TN removal can be 50.95%, the nitrogen removal can be 80.25%. It is therefore demonstrated that the strain A14 can be used to treat micro polluted water.