CAJ | 학술논문

背景：体外扩增的自体脂肪来源干细胞具有多向分化的潜能，在组织工程和再生医学领域具有广阔的应用前景。目的：观察自体脂肪来源干细胞移植受损人鼻黏膜组织的结构和功能变化。方法：选取因物理或化学因素造成鼻黏膜损害的患者10例，其中6例黏膜瘢痕，4例黏膜破溃，抽取自体脂肪组织进行脂肪来源干细胞体外分离、培养、扩增，移植前进行质量安全性检测。6例患者在黏膜瘢痕基底部、4例患者在黏膜破溃处注射移植脂肪来源干细胞(1×107/cm2结果与结论：脂肪来源干细胞注射移植后，所有患者临床症状得到缓解。与移植前比较，脂肪来源干细胞注射移植后30，90，150 d鼻腔容积及鼻腔最小横断面积有所减少(P <0.05)，鼻黏膜纤毛清除功能有所改善，但差异无显著性意义(P >0.05)。与移植前相比，脂肪来源干细胞移植30 d时3例患者鼻黏膜炎症明显减轻，胶原纤维排列整齐、沉积减少，杯状细胞分泌的黏蛋白增多。结果证明了体外扩增的脂肪来源干细胞可以重建鼻黏膜结构和恢复其功能。)，共注射3次，每2次间隔15 d。末次注射移植后30，90，150 d分别行鼻腔容积、最小横断面积、黏膜纤毛清除功能测定，其中3例患者在移植前和移植后30 d，取0.1 cm×0.1 cm黏膜组织，进行苏木精-伊红染色、Masson三色染色、AB-PAS染色。
배경：체외확증적자체지방래원간세포구유다향분화적잠능，재조직공정화재생의학영역구유엄활적응용전경。목적：관찰자체지방래원간세포이식수손인비점막조직적결구화공능변화。방법：선취인물리혹화학인소조성비점막손해적환자10례，기중6례점막반흔，4례점막파궤，추취자체지방조직진행지방래원간세포체외분리、배양、확증，이식전진행질량안전성검측。6례환자재점막반흔기저부、4례환자재점막파궤처주사이식지방래원간세포(1×107/cm2결과여결론：지방래원간세포주사이식후，소유환자림상증상득도완해。여이식전비교，지방래원간세포주사이식후30，90，150 d비강용적급비강최소횡단면적유소감소(P <0.05)，비점막섬모청제공능유소개선，단차이무현저성의의(P >0.05)。여이식전상비，지방래원간세포이식30 d시3례환자비점막염증명현감경，효원섬유배렬정제、침적감소，배상세포분비적점단백증다。결과증명료체외확증적지방래원간세포가이중건비점막결구화회복기공능。)，공주사3차，매2차간격15 d。말차주사이식후30，90，150 d분별행비강용적、최소횡단면적、점막섬모청제공능측정，기중3례환자재이식전화이식후30 d，취0.1 cm×0.1 cm점막조직，진행소목정-이홍염색、Masson삼색염색、AB-PAS염색。
BACKGROUND:Theex-vivo expanded autologous adipose-derived stem cels have the capability of multipotential differentiation and have a broad application prospect in the field of tissue engineering and regenerative medicine. OBJECTIVE:To observe the nasal mucosal structural repair and functional reconstruction usingex-vivo expanded autologous adipose-derived stem cels. METHODS:Ten patients with mucosal damage due to the physical or chemical factors were enroled, including six cases of mucosal scar and four cases of mucosal ulceration. Autologous adipose tissue was extracted forin vitro isolation, culture and expansion of adipose-derived stem cels. Before transplantation, quality safety testing was done. Al the patients were injected adipose-derived stem cels (1×107/cm2 0.1 cm mucosal tissue sample at 30 days before and after transplantation for hematoxylin-eosin staining, Masson ) at an interval of 15 days, totaly for three times. Nasal volume, minimum cross-sectional area, and mucociliary clearance function were determined at 30, 90, 150 days after the final injection. Three of 10 patients were selected to take a 0.1 cm× trichrome staining, and AB-PAS staining. RESULTS AND CONCLUSION:Clinical symptoms were aleviated in al patients undergoing transplantation of adipose-derived stem cels. Compared with the baseline data, the nasal volume and minimum cross-sectional area were both decreased at 30, 90, 150 days after transplantation (P < 0.05), and the mucociliary clearance function was improved but not significantly (P > 0.05). Compared with the baseline data, the inflammation of the nasal mucosa was significantly reduced, colagen fibers arranged neatly, the deposition was decreased, and mucin secreted from goblet cels was increased in the selected three patients at 30 days after cel transplantation. These findings indicate thatex-vivo expanded autologous adipose-derived stem cels can be used to reconstruct the nasal mucosal structure and its function.