CAJ | 학술논문

背景：关节软骨损伤后自我修复能力较弱，主要是由于其缺乏滋养血管并且细胞代谢缓慢等组织特性，目前的治疗方法都不能恢复软骨组织的原有功能，近年来软骨组织工程已引起了越来越多的关注。目的：观察Ⅰ型胶原海绵支架搭载骨形态发生蛋白14基因转染脂肪干细胞修复兔膝关节软骨损伤的效果。方法：取兔皮下脂肪组织分离培养脂肪干细胞，用腺病毒真核表达载体 Ad-CMV-BMP-14-IRES-hrGFP-1转染脂肪干细胞。Ⅰ型胶原海绵支架搭载转染后的脂肪干细胞，待细胞吸附后对兔膝关节全层软骨缺损进行修复。术后12周取手术关节，从大体方面、组织学方面综合评估缺损修复状况。结果与结论：骨形态发生蛋白14转染后的脂肪干细胞骨形态发生蛋白14和Ⅱ型胶原蛋白表达及Sox-9基因表达明显高于普通脂肪干细胞。术后12周，支架搭载经骨形态发生蛋白14转染的脂肪干细胞组软骨组织修复良好，平整光滑，光洁度、质地及颜色良好，交界区整合良好。支架搭载脂肪干细胞组软骨组织部分修复，有正常软骨光泽，质地与颜色接近正常，修复组织与正常软骨组织界限明显。单纯支架组几乎崩解塌陷，未见透明样软骨结构形成。结果可见腺病毒携带骨形态发生蛋白14基因转染后脂肪干细胞修复软骨缺损的能力有大幅提升。
배경：관절연골손상후자아수복능력교약，주요시유우기결핍자양혈관병차세포대사완만등조직특성，목전적치료방법도불능회복연골조직적원유공능，근년래연골조직공정이인기료월래월다적관주。목적：관찰Ⅰ형효원해면지가탑재골형태발생단백14기인전염지방간세포수복토슬관절연골손상적효과。방법：취토피하지방조직분리배양지방간세포，용선병독진핵표체재체 Ad-CMV-BMP-14-IRES-hrGFP-1전염지방간세포。Ⅰ형효원해면지가탑재전염후적지방간세포，대세포흡부후대토슬관절전층연골결손진행수복。술후12주취수술관절，종대체방면、조직학방면종합평고결손수복상황。결과여결론：골형태발생단백14전염후적지방간세포골형태발생단백14화Ⅱ형효원단백표체급Sox-9기인표체명현고우보통지방간세포。술후12주，지가탑재경골형태발생단백14전염적지방간세포조연골조직수복량호，평정광활，광길도、질지급안색량호，교계구정합량호。지가탑재지방간세포조연골조직부분수복，유정상연골광택，질지여안색접근정상，수복조직여정상연골조직계한명현。단순지가조궤호붕해탑함，미견투명양연골결구형성。결과가견선병독휴대골형태발생단백14기인전염후지방간세포수복연골결손적능력유대폭제승。
BACKGROUND:The articular cartilage has weak self-repair ability, mainly due to its lack of trophoblast cels in blood vessels and slow cel metabolism. Current treatment methods cannot restore the original function of the cartilage tissue, and cartilage tissue engineering in recent years has garnered increasing attention. OBJECTIVE:To observe the effect of adipose-derived stem cels transfected with bone morphogenetic protein 14 combined with type I colagen sponge scaffold on the repair of articular cartilage injury in the knee of rabbits. METHODS: Adipose-derived stem cels were isolated and cultured from rabbit subcutaneous adipose tissue, and transfected with Ad-CMV-BMP-14-IRES-hrGFP-1. Type I colagen sponge scaffold with the transfected adipose-derived stem cels was used to repair articular cartilage injury in the knee of rabbits. Twelve weeks after operation, the articular tissue was taken for gross assessment and histological evaluation. RESULTS AND CONCLUSION: The expressions of bone morphogenetic protein 14, type II colagen and Sox-9 were higher in cels transfected with bone morphogenetic protein 14 than untransfected ones. At 12 weeks after operation, adipose-derived stem cels transfected with bone morphogenetic protein 14 combined with type I colagen sponge scaffold had good repair effect on articular cartilage injuries, and the injured cartilage tissues were smooth and had good texture, color and integration junction; adipose-derived stem cels combined with type I colagen sponge scaffold could partialy repair the injured cartilage tissues that had similar color and texture to normal tissues, and there was a remarkable boundary between the repaired tissue and normal cartilage tissue;simple type I colagen sponge scaffold was almost colapsed, and no hyaline cartilage tissue formed. These findings indicate that transfection of bone morphogenetic protein 14 can strengthen the ability of adipose-derived stem cels dramaticaly to repair cartilage injuries.