CAJ | 학술논문

背景：自体骨髓间充质干细胞移植治疗心血管疾病已经成为当前研究的热点之一，但是患者自体骨髓间充质干细胞的增殖能力及向心肌样细胞方向分化的能力是否随年龄的改变而有所变化尚不清楚。目的：探讨大鼠骨髓间充质干细胞生物学特性的增龄性差异。方法：纯化培养不同年龄组 SD 大鼠骨髓间充质干细胞，流式细胞仪分析其细胞周期，同时骨髓间充质干细胞与乳鼠心肌细胞共培养，细胞免疫荧光技术分析骨髓间充质干细胞心肌肌钙蛋白T的表达。结果与结论：流式细胞仪分析显示处于G0/G1期骨髓间充质干细胞的百分率随年龄的增加而增加；骨髓间充质干细胞的肌钙蛋白T表达率亦随年龄的增加而减少。结果表明大鼠骨髓间充质干细胞的增殖与定向分化能力随着年龄的增长而下降。
배경：자체골수간충질간세포이식치료심혈관질병이경성위당전연구적열점지일，단시환자자체골수간충질간세포적증식능력급향심기양세포방향분화적능력시부수년령적개변이유소변화상불청초。목적：탐토대서골수간충질간세포생물학특성적증령성차이。방법：순화배양불동년령조 SD 대서골수간충질간세포，류식세포의분석기세포주기，동시골수간충질간세포여유서심기세포공배양，세포면역형광기술분석골수간충질간세포심기기개단백T적표체。결과여결론：류식세포의분석현시처우G0/G1기골수간충질간세포적백분솔수년령적증가이증가；골수간충질간세포적기개단백T표체솔역수년령적증가이감소。결과표명대서골수간충질간세포적증식여정향분화능력수착년령적증장이하강。
BACKGROUND:Autologous bone marrow mesenchymal stem cel transplantation for the treatment of cardiovascular disease has become one of the hotspots, but it is unclear whether the proliferation and directional differentiation of autologous bone marrow mesenchymal stem cels varies changes with age. OBJECTIVE: To explore the proliferation and differentiation changes of rat bone mesenchymal stem cels in different ages. METHODS:The bone marrow mesenchymal stem cels from Sprague-Dawley rats in different age groups were purified and cultured, and then examined by flow cytometry in terms of cel cycle. Meanwhile, neonatal rat cardiomyocytes were co-cultured with bone marrow mesenchymal stem cels. The morphologic changes of bone marrow mesenchymal stem cels and the protein expression of troponin T were detected with immunofluorescence technique. RESULTS AND CONCLUSION: The percentage of bone marrow mesenchymal stem cels in G0/G1 phase was increased with age; while the percentage of expression of troponin T proteins-positive bone marrow mesenchymal stem cels were decreased with age. These findings indicate that the proliferation and differentiation abilities of rat bone marrow mesenchymal stem cels descend with age.