CAJ | 학술논문

背景：干细胞移植治疗心肌梗死的具体机制一直不十分清楚。目的：探讨H2O2方法：选取24只9周龄C57BL/6小鼠随机分为心肌梗死组、胚胎干细胞组、H体外预处理小鼠胚胎干细胞对治疗心肌梗死疗效的影响。2O2预处理胚胎干细胞组，每组8只，所有小鼠制备心肌梗死模型1周后，分别于尾静脉分别注入150μL的PBS、150μL的胚胎干细胞(1×106)、150μL的H2O2预处理胚胎干细胞(1×106结果与结论：与心肌梗死组比较，胚胎干细胞组和H )。移植后第3周超声测量各组小鼠左心室舒张末内径、左心室收缩末内径和左心室射血分数。超声心动图检查后取心肌组织行天狼星红染色检测心肌胶原含量，计算平均胶原容积分数。2O2预处理胚胎干细胞组左心室舒张末内径和左心室收缩末内径明显下降，左心室射血分数明显升高(P<0.05)，全心质量/体质量、左心室质量/体质量、平均胶原容积分数明显降低(P <0.05)；与胚胎干细胞组比较，H2O2预处理胚胎干细胞组小鼠左心室舒张末内径和左心室收缩末内径明显下降，左心室射血分数明显升高(P<0.05)，全心质量/体质量、左心室质量/体质量、平均胶原容积分数明显降低(P<0.05)。所以体外H2O2预处理胚胎干细胞减轻了心肌梗死后心肌纤维化，心功能改善更为明显。
배경：간세포이식치료심기경사적구체궤제일직불십분청초。목적：탐토H2O2방법：선취24지9주령C57BL/6소서수궤분위심기경사조、배태간세포조、H체외예처리소서배태간세포대치료심기경사료효적영향。2O2예처리배태간세포조，매조8지，소유소서제비심기경사모형1주후，분별우미정맥분별주입150μL적PBS、150μL적배태간세포(1×106)、150μL적H2O2예처리배태간세포(1×106결과여결론：여심기경사조비교，배태간세포조화H )。이식후제3주초성측량각조소서좌심실서장말내경、좌심실수축말내경화좌심실사혈분수。초성심동도검사후취심기조직행천랑성홍염색검측심기효원함량，계산평균효원용적분수。2O2예처리배태간세포조좌심실서장말내경화좌심실수축말내경명현하강，좌심실사혈분수명현승고(P<0.05)，전심질량/체질량、좌심실질량/체질량、평균효원용적분수명현강저(P <0.05)；여배태간세포조비교，H2O2예처리배태간세포조소서좌심실서장말내경화좌심실수축말내경명현하강，좌심실사혈분수명현승고(P<0.05)，전심질량/체질량、좌심실질량/체질량、평균효원용적분수명현강저(P<0.05)。소이체외H2O2예처리배태간세포감경료심기경사후심기섬유화，심공능개선경위명현。
BACKGROUND:Specific mechanism of stem cel transplantation for treatment of myocardial infarction has been not very clear. OBJECTIVE:To investigate the effect of H2O2 METHODS: Twenty-four C57BL/6 mice, 9 weeks old, were randomized into myocardial infarction group, embryonic stem cels group, H-pretreated mouse embryonic stem cels in vitro for treatment of myocardial infarction. 2O2 pretreatment group, with eight mice in each group, which were respectively injected 150 μL PBS, 150 μL embryonic stem cels (1×106), and 150 μL H2O2-pretreated embryonic stem cels (1×106 RESULTS AND CONCLUSION:Compared with the myocardial infarction group, the left ventricular end-diastolic diameter and left ventricular end-systolic inner diameter were significantly decreased in the embryonic stem cels ) at 1 week after modeling. At 3 weeks after transplantation, left ventricular end-diastolic diameter, left ventricular end-systolic inner diameter and left ventricular ejection fraction of mice in each group were measured by ultrasound. Myocardial colagen content was detected by Sirius red staining and the average colagen volume fraction was calculated. group and H2O2 pretreatment group, while the left ventricular ejection fraction was significantly increased (P < 0.05). Heart weight/body weight, left ventricular weight/body weight, and average colagen volume fraction were significantly lower in the embryonic stem cels group and H2O2 pretreatment group than the myocardial infarction group (P < 0.05). Therefore,in vitro H2O2 pretreatment enhances reduce the post-myocardial infarction myocardial fibrosis and greatly improve heart function.