CAJ | 학술논문

目的：研究异粘蛋白（ MTDH）在胃癌的表达及在胃癌转移中的作用。方法收集临床胃癌标本及培养胃癌细胞系，采用Western blot 和Real－time PCR的方法检测组织中MTDH蛋白与mRNA表达水平及与EMT的关系。结果胃癌组织中187例（86％） MTDH呈阳性表达，正常胃黏膜组织中29例（13％）呈阳性表达，MTDH在胃癌中的表达水平比正常组织高。 MTDH蛋白表达与胃癌患者的TNM分期、血管浸润、肿瘤复发和转移有关（P＜0．05）。 MTDH的表达与EMT过程的两个标记物相关（E－cadherin和N－cadherin ）。结论 MTDH可以通过诱导EMT过程促进胃癌转移，可以作为临床治疗的一个靶基因。
목적：연구이점단백（ MTDH）재위암적표체급재위암전이중적작용。방법수집림상위암표본급배양위암세포계，채용Western blot 화Real－time PCR적방법검측조직중MTDH단백여mRNA표체수평급여EMT적관계。결과위암조직중187례（86％） MTDH정양성표체，정상위점막조직중29례（13％）정양성표체，MTDH재위암중적표체수평비정상조직고。 MTDH단백표체여위암환자적TNM분기、혈관침윤、종류복발화전이유관（P＜0．05）。 MTDH적표체여EMT과정적량개표기물상관（E－cadherin화N－cadherin ）。결론 MTDH가이통과유도EMT과정촉진위암전이，가이작위림상치료적일개파기인。
Objective To explore the relationship between gene MTDH expression and its role in promo-ting gastric carcinoma metastasis .Methods We collected clinical specimens and cultured gastric carcinoma cell lines.By Western blotting and Real -time PCR methods,protein and mRNA levels in tissues and MTDH relation-ship with EMT were detected .Results There was 86%of patients who expressed MTDH positively and 13%of normal gastric mucosa was positive expression .The results showed that the expressive level of MTDH gene in gas-tric carcinoma was higher than in the normal tissues .The expression of MTDH was correlated with TNM stage、mi-crovascular invasion、recurrence and metastasis .The expressive level of MTDH was correlated with two epithelial mesenchymal transition markers ( E-cadherin and N-cadherin ) .Conclusion MTDH may promote gastric car-cinoma metastasis through the induction of EMT process and may be a candidate biomarker for therapeutic target .