CAJ | 학술논문

背景：骨髓基质干细胞移植到梗死心肌组织能够起到抑制和减少心肌细胞凋亡的效应，而这一作用与心功能改善是否有相关性还不清楚。目的：观察缺血心肌内移植骨髓基质干细胞早期对心脏功能的影响。方法：采用结扎左前降支的方法建立大鼠急性心肌梗死模型，假手术组仅穿线不结扎。骨髓基质干细胞移植组于心肌梗死术后30 min分5个位置于梗死边缘向心肌组织内移植大鼠骨髓基质干细胞0.1 mL(2×106结果与结论：移植后第3天，与假手术组相比，模型组梗死区及缺血区心肌细胞凋亡明显；骨髓基质干细胞移植组梗死区和缺血区心肌细胞凋亡数目均较模型组显著减少。与假手术组相比，模型组和骨髓基质干细胞移植组大鼠平均动脉压、左心室收缩压明显下降，左心室舒张末压显著升高，左心室射血分数及缩短分数显著降低(P <0.05)，骨髓基质干细胞移植组和模型组比较差异无显著性意义(P >0.05)。结果表明骨髓基质干细胞移植早期能减少心肌细胞凋亡但不改善急性心肌梗死后大鼠的心功能。假手术组、模型组术后分别向心肌内注射相同剂量的生理盐水。细胞移植后3 d监测血流动力学、超声心动图， TUNEL法检测心肌细胞凋亡的变化。
배경：골수기질간세포이식도경사심기조직능구기도억제화감소심기세포조망적효응，이저일작용여심공능개선시부유상관성환불청초。목적：관찰결혈심기내이식골수기질간세포조기대심장공능적영향。방법：채용결찰좌전강지적방법건립대서급성심기경사모형，가수술조부천선불결찰。골수기질간세포이식조우심기경사술후30 min분5개위치우경사변연향심기조직내이식대서골수기질간세포0.1 mL(2×106결과여결론：이식후제3천，여가수술조상비，모형조경사구급결혈구심기세포조망명현；골수기질간세포이식조경사구화결혈구심기세포조망수목균교모형조현저감소。여가수술조상비，모형조화골수기질간세포이식조대서평균동맥압、좌심실수축압명현하강，좌심실서장말압현저승고，좌심실사혈분수급축단분수현저강저(P <0.05)，골수기질간세포이식조화모형조비교차이무현저성의의(P >0.05)。결과표명골수기질간세포이식조기능감소심기세포조망단불개선급성심기경사후대서적심공능。가수술조、모형조술후분별향심기내주사상동제량적생리염수。세포이식후3 d감측혈류동역학、초성심동도， TUNEL법검측심기세포조망적변화。
BACKGROUND:Bone marrow stromal stem cels transplanted into infracted cardiac tissue can inhibit and reduce myocardial apoptosis, but whether this effect is correlated with improvement in cardiac function is stil unclear. OBJECTIVE:To study the early effect of bone marrow stromal stem cels transplanted into the ischemic myocardium on the cardiac function. METHODS: Models of acute myocardial infarction were established by ligation of the left anterior descending branch, while no ligation was done in the sham group. In the transplantation group, rat bone marrow stromal stem cels (0.1 mL, 2×106 RESULTS AND CONCLUSION:At 3 days after cel transplantation, myocardial apoptosis was more evident in the infarct and ischemic zones of the model group than the sham group; the number of apoptotic myocardial cels was significantly lower in the infarct and ischemic zones of the transplantation group than the model group. Compared with the sham group, the mean arterial blood pressure and left ventricular systolic pressure were ) were injected into five sites on the edge of infarcted myocardial tissues at 30 minutes after myocardial infarction. In the sham group and model group, the same volume of normal saline was injected into the myocardial tissues. Three days after cel transplantation, hemodynamic monitoring, echocardiography, TUNEL assay were employed to detect myocardial apoptosis. significantly reduced, the left ventricular end diastolic pressure was increased, and the left ventricular ejection fraction and shortened fraction were significantly lowered in the model and transplantation groups (P < 0.05), but there was no difference between the model and transplantation groups (P > 0.05). These findings indicate that myocardial apoptosis can be reduced but the cardiac function cannot be improved in acute myocardial infarction rats at early stage after bone marrow stromal stem cels transplantation.