CAJ | 학술논문

背景：研究显示芍药苷具有补血及治疗自身免疫性疾病的功效，骨髓间充质干细胞对机体的造血及免疫功能也起着重要的作用，但芍药苷对骨髓间充质干细胞的增殖及细胞因子的分泌和表达有何影响报道较少。目的：探讨芍药苷对人骨髓间充质干细胞增殖及白细胞介素6表达的影响。方法：采用密度梯度离心法和贴壁培养法体外分离培养人骨髓间充质干细胞，用流式细胞术和成脂及成骨诱导法鉴定人骨髓间充质干细胞生物学特性， MTT法检测不同浓度芍药苷对人骨髓间充质干细胞增殖的影响， EL ISA 法测定芍药苷干预人骨髓间充质干细胞后培养上清液中白细胞介素6的分泌水平，RT-PCR 检测芍药苷干预后白细胞介素6 mRNA的表达情况。结果与结论：成功分离出骨髓间充质干细胞，具有成骨、成脂分化潜能。与对照组相比，芍药苷浓度为2μmol/L和10μmo l/L可明显促进骨髓间充质干细胞增殖。10μmol/L 芍药苷干预骨髓间充质干细胞后， G0/G1期细胞比例显著降低， S期细胞比例显著升高。10μmol/L 芍药苷干预组骨髓间充质干细胞白细胞介素6 的分泌和mRNA表达均显著高于对照组(P <0.01)。由此得出，一定浓度的芍药苷可促进骨髓间充质干细胞增殖，并提高骨髓间充质干细胞分泌白细胞介素6水平和基因表达。
배경：연구현시작약감구유보혈급치료자신면역성질병적공효，골수간충질간세포대궤체적조혈급면역공능야기착중요적작용，단작약감대골수간충질간세포적증식급세포인자적분비화표체유하영향보도교소。목적：탐토작약감대인골수간충질간세포증식급백세포개소6표체적영향。방법：채용밀도제도리심법화첩벽배양법체외분리배양인골수간충질간세포，용류식세포술화성지급성골유도법감정인골수간충질간세포생물학특성， MTT법검측불동농도작약감대인골수간충질간세포증식적영향， EL ISA 법측정작약감간예인골수간충질간세포후배양상청액중백세포개소6적분비수평，RT-PCR 검측작약감간예후백세포개소6 mRNA적표체정황。결과여결론：성공분리출골수간충질간세포，구유성골、성지분화잠능。여대조조상비，작약감농도위2μmol/L화10μmo l/L가명현촉진골수간충질간세포증식。10μmol/L 작약감간예골수간충질간세포후， G0/G1기세포비례현저강저， S기세포비례현저승고。10μmol/L 작약감간예조골수간충질간세포백세포개소6 적분비화mRNA표체균현저고우대조조(P <0.01)。유차득출，일정농도적작약감가촉진골수간충질간세포증식，병제고골수간충질간세포분비백세포개소6수평화기인표체。
BACKGROUND:Studies have shown that paeoniflorin functions as replenishing blood and treatment of autoimmune diseases, and bone marrow mesenchymal stem cels also play an important role in the body’s blood and immune function. However, paeoniflorin effects on bone marrow mesenchymal stem cel proliferation and cytokine secretion and expression are rarely reported. OBJECTIVE:To investigate the effect of paeoniflorin on proliferation of bone marrow mesenchymal stem cels and the expression of interleukin-6. METHODS:Human bone marrow mesenchymal stem cels were separated and culturedin vitro by density gradient centrifugation combined with attachment method. The biological characteristics of bone marrow mesenchymal stem cels were identified by flow cytometry and osteogenic/adipogenic induction. The proliferation of bone marrow mesenchymal stem cels under different concentrations of paeoniflorin was detected by MTT method. The mRNA expression and secretion of interleukin-6 in the supernatant of bone marrow mesenchymal stem cels were detected by RT-PCR and ELISA, respectively. RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cels were isolated successfuly and had osteogenic and adipogenic differentiation potential. Compared with the controlgroup, 2 μmol/L and 10 μmol/L paeoniflorin significantly promoted the proliferation of bone marrow mesenchymal stem cels. 10 μmol/L paeoniflorin could significantly decrease the proportion of bone marrow mesenchymal stem cels in G0/G1 phase and increase this proportion in S phase. Compared with the control group, the experimental group could significantly increase the secretion and mRNA expression of interleukin-6 (P < 0.01). It is concluded that paeoniflorin at certain concentrations can obviously promote the proliferation of bone marrow mesenchymal stem cels, and increase the expression and secretion of interleukin-6.