微循环学杂志
微循環學雜誌
미순배학잡지
CHINESE JOURNAL OF MICROCIRCULATION
2015年
1期
37-41
,共5页
谢海龙%李俊立%万靖%李承彬
謝海龍%李俊立%萬靖%李承彬
사해룡%리준립%만정%리승빈
2 型糖尿病%颈动脉粥样硬化%外周血单个核细胞%凋亡相关斑点样蛋白
2 型糖尿病%頸動脈粥樣硬化%外週血單箇覈細胞%凋亡相關斑點樣蛋白
2 형당뇨병%경동맥죽양경화%외주혈단개핵세포%조망상관반점양단백
Type 2 diabetes mellitus%Carotid atherosclerosis%Peripheral blood mononuclear cells%Apoptosis-associated speck-like protein containing a CARD
目的：：分析2型糖尿病(T2DM)合并颈动脉粥样硬化(CAS)患者外周血单个核细胞(PBMCs)中衔接蛋白凋亡相关斑点样蛋白(ASC)mRNA 的表达及作用。方法：根据华盛顿大学 CAS 斑块超声分级标准将107例T2DM 患者分为单纯 T2DM 组(n=26)、T2DM 轻度斑块组(n=32)、T2DM 中度斑块组(n=38)和 T2DM 重度斑块组(n=11)。另选非 T2DM 的 CAS 患者作为单纯 CAS 组(n =35),以及体检健康者作为正常对照组(n =35)。采用实时荧光定量聚合酶链反应(RT-FQ-PCR)技术检测各组 PBMCs 中 ASC mRNA 表达水平。统计分析各组差异及 ASC mRNA 水平与 CAS 斑块严重程度的关系。结果：各病例组 ASC mRNA 表达水平显著高于正常对照组(P <0.05),T2DM 合并 CAS 组 ASC mRNA 表达水平显著高于单纯 CAS 组(P <0.01)；ASC mRNA 表达水平轻度斑块组>中度斑块组>重度斑块组,即 ASC mRNA 表达水平与 T2DM 患者 CAS 斑块程度呈明显负相关(r=-0.43,P <0.05)。结论：ASC mRNA 表达增加可能是 T2DM 合并 AS 的发病因素和 CAS 斑块活跃性的指征。
目的：：分析2型糖尿病(T2DM)閤併頸動脈粥樣硬化(CAS)患者外週血單箇覈細胞(PBMCs)中銜接蛋白凋亡相關斑點樣蛋白(ASC)mRNA 的錶達及作用。方法：根據華盛頓大學 CAS 斑塊超聲分級標準將107例T2DM 患者分為單純 T2DM 組(n=26)、T2DM 輕度斑塊組(n=32)、T2DM 中度斑塊組(n=38)和 T2DM 重度斑塊組(n=11)。另選非 T2DM 的 CAS 患者作為單純 CAS 組(n =35),以及體檢健康者作為正常對照組(n =35)。採用實時熒光定量聚閤酶鏈反應(RT-FQ-PCR)技術檢測各組 PBMCs 中 ASC mRNA 錶達水平。統計分析各組差異及 ASC mRNA 水平與 CAS 斑塊嚴重程度的關繫。結果：各病例組 ASC mRNA 錶達水平顯著高于正常對照組(P <0.05),T2DM 閤併 CAS 組 ASC mRNA 錶達水平顯著高于單純 CAS 組(P <0.01)；ASC mRNA 錶達水平輕度斑塊組>中度斑塊組>重度斑塊組,即 ASC mRNA 錶達水平與 T2DM 患者 CAS 斑塊程度呈明顯負相關(r=-0.43,P <0.05)。結論：ASC mRNA 錶達增加可能是 T2DM 閤併 AS 的髮病因素和 CAS 斑塊活躍性的指徵。
목적：：분석2형당뇨병(T2DM)합병경동맥죽양경화(CAS)환자외주혈단개핵세포(PBMCs)중함접단백조망상관반점양단백(ASC)mRNA 적표체급작용。방법：근거화성돈대학 CAS 반괴초성분급표준장107례T2DM 환자분위단순 T2DM 조(n=26)、T2DM 경도반괴조(n=32)、T2DM 중도반괴조(n=38)화 T2DM 중도반괴조(n=11)。령선비 T2DM 적 CAS 환자작위단순 CAS 조(n =35),이급체검건강자작위정상대조조(n =35)。채용실시형광정량취합매련반응(RT-FQ-PCR)기술검측각조 PBMCs 중 ASC mRNA 표체수평。통계분석각조차이급 ASC mRNA 수평여 CAS 반괴엄중정도적관계。결과：각병례조 ASC mRNA 표체수평현저고우정상대조조(P <0.05),T2DM 합병 CAS 조 ASC mRNA 표체수평현저고우단순 CAS 조(P <0.01)；ASC mRNA 표체수평경도반괴조>중도반괴조>중도반괴조,즉 ASC mRNA 표체수평여 T2DM 환자 CAS 반괴정도정명현부상관(r=-0.43,P <0.05)。결론：ASC mRNA 표체증가가능시 T2DM 합병 AS 적발병인소화 CAS 반괴활약성적지정。
Objective:To analysis expression and role of adaptor protein apoptosis-associated speck-like pro-tein containing a CARD (ASC)mRNA in peripheral blood mononuclear cells (PBMCs)of type 2 diabetes mellitus (T2DM)merger with carotid atherosclerosis (CAS)patient.Method:107 T2DM patients were enrolled,divided them into T2DM group (n = 26 ),T2DM small plaque group (n = 32 ),T2DM moderate plaque group (n = 38 ), T2DM serious plaque group (n = 1 1 )according to atherosclerosis plaque ultrasonic classification constituded by Washington university.Another non T2DM CAS patients as CAS group (n = 35 ),and healthy persons as normal control group (n=35).The ASC mRNA expression levels of PBMCs in each group was deteeted by real-time fluo-rescent quantitative polymerase chain reaction (RT-FQ-PCR)technology.Statistical analysis of the differences be-tween each group and relationship between ASC mRNA levels and the severity of CAS patches.Results:Expression level of ASC mRNA was significantly higher in the pathological group than normal control group (P <0.05 ),ex-pression level of ASC mRNA was significantly higher in T2DM combined with CAS group than CAS group (P <0. 01),ASC mRNA expression in small plaque group greater than moderate plaque group and severe plaque group,ex-pression of ASC mRNA was negatively correlated with CAS plaque degree in T2DM patients(r=-0.43,P <0.05). Conclusion:Increased expression of ASC mRNA may be risk factor of T2DM combined with AS and CAS.
