微循环学杂志
微循環學雜誌
미순배학잡지
CHINESE JOURNAL OF MICROCIRCULATION
2015年
1期
14-17
,共4页
细胞穿透肽 PEP-1%血红素加氧酶 1%肝脏缺血再灌注损伤%大鼠
細胞穿透肽 PEP-1%血紅素加氧酶 1%肝髒缺血再灌註損傷%大鼠
세포천투태 PEP-1%혈홍소가양매 1%간장결혈재관주손상%대서
PEP-1%HO-1%Hepar ischemia-reperfusion injury%Rat
目的::探讨细胞穿透肽 PEP-1介导的血红素加氧酶-1(HO-1)对大鼠肝脏缺血再灌注损伤(HIRI)的保护作用。方法:(1)用基因工程学方法人工合成融合蛋白 PEP-1-HO-1。(2)选择雄性 SD 大鼠,随机分为三组(n均=8):假手术组(S 组)只开腹,不予干预。HIRI 模型组(HIRI 组),采用夹闭肝动脉和门静脉30min 后恢复血流;HIRI+PEP-1-HO-1预处理组(HIRI+HO-1组),夹闭肝动脉和门静脉前经门静脉注射 PEP-1-HO-1蛋白1mg,其余处理同 HIRI 组。(3)实验完成后,取三组大鼠下腔静脉血,采用自动生化分析仪测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、γ-谷氨酰转肽酶(γ-GT)、总胆红素(TBIL)等肝功能指标。处死大鼠,取部分肝脏进行组织切片、HE 染色,观察各组肝组织病理学变化。结果:成功制备高纯度 PEP-1-HO-1融合蛋白。HIRI 组大鼠各项肝功能指标均显著高于 S 组(P <0.01),HIRI+HO-1组各项肝功能指标值明显低于 HIRI 组(P <0.01),但仍高于 S 组(P <0.05)。HIRI 组肝细胞肿大或呈球形,胞浆疏松水样变或完全透明,伴炎细胞浸润,可见片状坏死。 HIRI+HO-1组肝脏损伤程度较 HIRI 组明显改善,炎性细胞浸润及肝细胞坏死程度明显减轻,但与 S 组比较,肝脏组织损伤仍明显。结论:用细胞穿透肽 PEP-1介导 HO-1预处理能有效保护肝细胞,明显减轻肝功能损害。
目的::探討細胞穿透肽 PEP-1介導的血紅素加氧酶-1(HO-1)對大鼠肝髒缺血再灌註損傷(HIRI)的保護作用。方法:(1)用基因工程學方法人工閤成融閤蛋白 PEP-1-HO-1。(2)選擇雄性 SD 大鼠,隨機分為三組(n均=8):假手術組(S 組)隻開腹,不予榦預。HIRI 模型組(HIRI 組),採用夾閉肝動脈和門靜脈30min 後恢複血流;HIRI+PEP-1-HO-1預處理組(HIRI+HO-1組),夾閉肝動脈和門靜脈前經門靜脈註射 PEP-1-HO-1蛋白1mg,其餘處理同 HIRI 組。(3)實驗完成後,取三組大鼠下腔靜脈血,採用自動生化分析儀測定血清穀丙轉氨酶(ALT)、穀草轉氨酶(AST)、γ-穀氨酰轉肽酶(γ-GT)、總膽紅素(TBIL)等肝功能指標。處死大鼠,取部分肝髒進行組織切片、HE 染色,觀察各組肝組織病理學變化。結果:成功製備高純度 PEP-1-HO-1融閤蛋白。HIRI 組大鼠各項肝功能指標均顯著高于 S 組(P <0.01),HIRI+HO-1組各項肝功能指標值明顯低于 HIRI 組(P <0.01),但仍高于 S 組(P <0.05)。HIRI 組肝細胞腫大或呈毬形,胞漿疏鬆水樣變或完全透明,伴炎細胞浸潤,可見片狀壞死。 HIRI+HO-1組肝髒損傷程度較 HIRI 組明顯改善,炎性細胞浸潤及肝細胞壞死程度明顯減輕,但與 S 組比較,肝髒組織損傷仍明顯。結論:用細胞穿透肽 PEP-1介導 HO-1預處理能有效保護肝細胞,明顯減輕肝功能損害。
목적::탐토세포천투태 PEP-1개도적혈홍소가양매-1(HO-1)대대서간장결혈재관주손상(HIRI)적보호작용。방법:(1)용기인공정학방법인공합성융합단백 PEP-1-HO-1。(2)선택웅성 SD 대서,수궤분위삼조(n균=8):가수술조(S 조)지개복,불여간예。HIRI 모형조(HIRI 조),채용협폐간동맥화문정맥30min 후회복혈류;HIRI+PEP-1-HO-1예처리조(HIRI+HO-1조),협폐간동맥화문정맥전경문정맥주사 PEP-1-HO-1단백1mg,기여처리동 HIRI 조。(3)실험완성후,취삼조대서하강정맥혈,채용자동생화분석의측정혈청곡병전안매(ALT)、곡초전안매(AST)、γ-곡안선전태매(γ-GT)、총담홍소(TBIL)등간공능지표。처사대서,취부분간장진행조직절편、HE 염색,관찰각조간조직병이학변화。결과:성공제비고순도 PEP-1-HO-1융합단백。HIRI 조대서각항간공능지표균현저고우 S 조(P <0.01),HIRI+HO-1조각항간공능지표치명현저우 HIRI 조(P <0.01),단잉고우 S 조(P <0.05)。HIRI 조간세포종대혹정구형,포장소송수양변혹완전투명,반염세포침윤,가견편상배사。 HIRI+HO-1조간장손상정도교 HIRI 조명현개선,염성세포침윤급간세포배사정도명현감경,단여 S 조비교,간장조직손상잉명현。결론:용세포천투태 PEP-1개도 HO-1예처리능유효보호간세포,명현감경간공능손해。
Objective:To study liver protection of PEP-1 mediated heme oxygenase 1(HO-1)in rat hepar is-chemia-reperfusion injury(HIRI).Method:(1 )Synthetic protein (PEP-1-HO-1 )was made by genetic engineering methods.(2)24 male SD rats were randomly assigned to three groups:the sham group(S group),HIRI model group (HIRI group),HIRI and PEP-1-HO-1 pretreatment group(HIRI+HO-1 group).(3)After experiment completely, extraction of three groups of inferior vena venous blood of rats by automatic biochemical analyzer determine serum:cereal third transominase (ALT),aspertate aminotransferase (AST),gamma glutamyl transpeptidase(γ-GT),total bilirubin (TBIL)and liver function indicators.Taking part executed in rats after the liver biopsy HE staining,each group liver tissue pathological changes were observed.Results:Successful preparation of high purity PEP-1-HO-1 fusion protein.Rats liver function index HIRI group were significantly higher than S group (P < 0.01 ),HIRI+HO-1 set of various liver function index was lower than that in group HIRI (P <0.01),but it was still higher than the group S (P <0.05).HIRI+HO-1 set of HIRI group significantly reduced degree of liver injury,inflammatory cell infiltration and necrosis of liver cells was significantly reduced,but compared with the group S ,the liver tissue damage was still obvious.Conclusion:It can pretreatment effectively protect the liver cells with cell penetrating pep-tides PEP-1 mediated heme oxygenase 1,significantly reduce liver damage.