CAJ | 학술논문

目的：：检测湖北地区丙型肝炎(丙肝)患者丙肝病毒(HCV)基因分型及 HCV NS5B 基因耐药突变位点的分布特征。方法：收集自2011-03-2014-05在本院确诊的来自湖北地区的丙肝患者外周静脉血标本273例，采用一代测序法检测每例样本的 HCV 和 NS5B 基因序列，将测得的序列与 Blast 进行在线比对后，统计分析 HCV基因型和各亚型检出率，以及 HCV NS5B 耐药突变位点在 HCV 各基因亚型中的分布差异。结果：273例丙肝患者共检出1、2、3、6四种基因型和1a、1b、2a、3a、3b、6a 六种基因亚型，其中1b 亚型检出率较高，占76.19%(208/273)，其次是2a 亚型，占15.02%(41/273)，其它各亚型检出率均不超过4.40%，各亚型检出率差异有统计学意义(P <0.05)。HCV NS5B 基因以 L159F 突变率较高，占17.95%(42/234)，与其它位点的基因突变率差异有统计学意义(P <0.05)。同时各个位点的耐药突变发生在2a 亚型中的比例较高，达57.26%(134/234)，显著高于其它亚型的耐药突变(P <0.05)。结论：分析湖北地区丙肝患者 HCV 基因分型及 HCV NS5B 耐药突变位点的分布特点，可为该地区丙肝患者的个体化治疗提供指导依据。
목적：：검측호북지구병형간염(병간)환자병간병독(HCV)기인분형급 HCV NS5B 기인내약돌변위점적분포특정。방법：수집자2011-03-2014-05재본원학진적래자호북지구적병간환자외주정맥혈표본273례，채용일대측서법검측매례양본적 HCV 화 NS5B 기인서렬，장측득적서렬여 Blast 진행재선비대후，통계분석 HCV기인형화각아형검출솔，이급 HCV NS5B 내약돌변위점재 HCV 각기인아형중적분포차이。결과：273례병간환자공검출1、2、3、6사충기인형화1a、1b、2a、3a、3b、6a 륙충기인아형，기중1b 아형검출솔교고，점76.19%(208/273)，기차시2a 아형，점15.02%(41/273)，기타각아형검출솔균불초과4.40%，각아형검출솔차이유통계학의의(P <0.05)。HCV NS5B 기인이 L159F 돌변솔교고，점17.95%(42/234)，여기타위점적기인돌변솔차이유통계학의의(P <0.05)。동시각개위점적내약돌변발생재2a 아형중적비례교고，체57.26%(134/234)，현저고우기타아형적내약돌변(P <0.05)。결론：분석호북지구병간환자 HCV 기인분형급 HCV NS5B 내약돌변위점적분포특점，가위해지구병간환자적개체화치료제공지도의거。
Objective:To detect genotypes of HCV and distribution characters of gene mutation loci of drug-resistant of HCV NS5B in hubei province.Method:Venous blood samples of 273 patients,since March 201 1 to May 2014 diagnosed in our hospital from hubei province,were collected and detected for HCV and NS5B gene sequence by generation sequencing assay.Then we compared detected sequences with BLAST online,and analyzed statistical-ly detection rates of each genotype and subtype of HCV and distribution differences of drug-resistant mutation loci of HCV NS5B.Results:There were 1,2,3 and 6 four kinds of genotypes and 1a,1b,2a,3a,3b and 6a six kinds of ge-netic subtypes in 273 cases.Of those,the detection rate of 1b was higher,accounting for 76.1 9%(208/273),fol-lowed by 2a subtype was 1 5.02% (41/273 ),the detection rate of other subtypes was not over 4.40%,and the difference of each subtype detection rates was statistically significant (χ2 =71 9.20,P <0.05).The gene mutation rate of L1 59F site in HCV NS5B was higher,accounting for 1 7.95% (42/234),compared with mutation rates of other sites,the difference was statistically significant (χ2 =272.32,P <0.05).While all the sites of gene mutations in HCV 2a subtype was in a higher proportion up to 57.26% (134/234),significantly higher than mutation rates of other subtypes(χ2 = 53.07,P < 0.05 ).Conclusion:Analysis of genotypes of HCV and distribution characters of drug-resistant mutation loci of HCV NS5B in hubei province could provide basis for individualized treatment in the area.