CAJ | 학술논문

目的：研究卵巢切除对SD 大鼠骨折骨痂形态的影响。方法雌性SD大鼠56只，随机分为两组，每组28只。实验组大鼠3月龄时切除双侧卵巢建立绝经后骨质疏松症模型，另一组行假手术对照。4周后建立双侧股骨骨折模型。分别在骨折前、骨折后6周、16周采用QCT及自动图像分析仪检测两组大鼠骨折区域骨痂情况，指标包括骨痂面积、骨痂组成成分、骨含量、骨密度、骨矿化面积、骨矿化率及骨生成率等。结果卵巢切除导致在不同时期大鼠股骨骨密度均降低，与对照组比较差异有统计学意义（P＜0?05）。骨折后6周实验组骨生成率、骨痂面积较对照组大，而骨矿化面积、骨矿化率与对照组无显著差异。实验组骨痂成分以编织骨为主，与对照组相比差异有统计学意义（ P＜0?05）。骨折后16周实验组骨痂面积及骨痂成分与对照组相比，差异无统计学意义（P＞0?05）；而破骨细胞表面及数量较对照组增加，差异有统计学意义（P＜0?05）。与对照组相比，实验组股骨髓腔面积增大，骨皮质面积减少，差异均有统计学意义（P＜0?05）。结论卵巢切除导致的高骨转换可能会加快骨痂改建速度，从而促进骨折愈合。
목적：연구란소절제대SD 대서골절골가형태적영향。방법자성SD대서56지，수궤분위량조，매조28지。실험조대서3월령시절제쌍측란소건립절경후골질소송증모형，령일조행가수술대조。4주후건립쌍측고골골절모형。분별재골절전、골절후6주、16주채용QCT급자동도상분석의검측량조대서골절구역골가정황，지표포괄골가면적、골가조성성분、골함량、골밀도、골광화면적、골광화솔급골생성솔등。결과란소절제도치재불동시기대서고골골밀도균강저，여대조조비교차이유통계학의의（P＜0?05）。골절후6주실험조골생성솔、골가면적교대조조대，이골광화면적、골광화솔여대조조무현저차이。실험조골가성분이편직골위주，여대조조상비차이유통계학의의（ P＜0?05）。골절후16주실험조골가면적급골가성분여대조조상비，차이무통계학의의（P＞0?05）；이파골세포표면급수량교대조조증가，차이유통계학의의（P＜0?05）。여대조조상비，실험조고골수강면적증대，골피질면적감소，차이균유통계학의의（P＜0?05）。결론란소절제도치적고골전환가능회가쾌골가개건속도，종이촉진골절유합。
Objective To investigate the effect of ovariectomy on the morphology of fracture healing callus in Sprague?Dawley rats. Methods Fifty?eight 3-month?old female Sprague?Dawley rats were randomly divided into 2 groups, with 28 rats in each group. Rats in the experiment group received bilateral ovariectomy ( OVX) to establish the osteoporosis model. Rats in sham group received sham operation, and received bilateral osteotomy in 4 weeks to establish the fracture model. The status of the fracture callus in both groups, including the area, the content, bone mass, bone mineral density, calcified area and rate, and bone formation rate, was analyzed using QCT before the fracture and 6?week and 16?week after the fracture. Results Ovariectomy resulted in the decrease of bone mineral density in the femur of the rats, and the difference was statistically significant comparing to the control group (P<0?05). After the 6 weeks of the fracture, the bone formation rate and the area of the calluses in OVX rats were higher and larger than those in sham rats, but the calcified area and rate were not different comparing to the control. The composition of calluses was mainly woven bone in the experiment group, which was significantly different to the sham group (P<0?05). After 16 weeks of the fracture, there was no difference in callus area and composition between the 2 groups ( P >0?05 ) . The osteoclast surface and number in OVX rats increased, with statistical significance (P<0?05). The bone marrow area increased and the cortical bone area decreased in OVX group comparing to those in sham group, and the differences were statistically significant (P<0?05). Conclusion OVX?stimulates bone turnover thus accelerates the progression of fracture healing.