CAJ | 학술논문

目的：了解小鼠在去势前与去势后，铁对骨吸收影响的差异。方法建立高铁小鼠模型，将小鼠随机分为假手术组（ SHAM组）、高铁去势组（ F＋OVX组）、去势组（ OVX组）。铁剂干预两周后去势。在去势前（雌激素存在）与去势7周后（雌激素缺乏）检测小鼠体重，血清铁蛋白；反转录聚合酶链反应（ RT?PCR）检测胫骨内抗酒石酸酸性磷酸酶（ TRAP）、降钙素受体（CTR）、基质金属蛋白酶9（MMP9）、组织蛋白酶K（cathepsin K）、雌激素受体α（ERα）、雌激素受体β（ERβ）表达情况，股骨远端Micro?CT三维分析，提取各组小鼠股骨骨髓细胞进行破骨细胞培养，抗酒石酸酸性磷酸酶（ TRAP）染色观察破骨细胞分化情况。结果各组小鼠体重无明显差异。 F＋OVX组铁蛋白含量明显升高（P＜0?05）。 Micro?CT分析表明：雌激素存在时，正常组与铁干预组骨密度及相关指标无明显差异（P＞0?05）；雌激素缺乏时，与OVX组比较，F＋OVX组骨量下降更为严重（P＜0?05）。半定量PCR结果显示：雌激素存在时，两组TRAP、CTR、MMP9、cathepsin K基因表达无统计学差异；雌激素缺乏时， F＋OVX组各基因表达水平较OVX组显著升高（P＜0?05）。去势后ERβ表达下降（P＜0?05），ERα无明显差异。 TRAP染色显示去势前各组小鼠破骨细胞数无明显差异（P＞0?05）；而去势后，F＋OVX组破骨细胞数明显高于OVX组（P＜0?05）。结论未去势时（有雌激素），FAC对骨吸收影响甚微；去势后（无雌激素），FAC能显著增强破骨活性。
목적：료해소서재거세전여거세후，철대골흡수영향적차이。방법건립고철소서모형，장소서수궤분위가수술조（ SHAM조）、고철거세조（ F＋OVX조）、거세조（ OVX조）。철제간예량주후거세。재거세전（자격소존재）여거세7주후（자격소결핍）검측소서체중，혈청철단백；반전록취합매련반응（ RT?PCR）검측경골내항주석산산성린산매（ TRAP）、강개소수체（CTR）、기질금속단백매9（MMP9）、조직단백매K（cathepsin K）、자격소수체α（ERα）、자격소수체β（ERβ）표체정황，고골원단Micro?CT삼유분석，제취각조소서고골골수세포진행파골세포배양，항주석산산성린산매（ TRAP）염색관찰파골세포분화정황。결과각조소서체중무명현차이。 F＋OVX조철단백함량명현승고（P＜0?05）。 Micro?CT분석표명：자격소존재시，정상조여철간예조골밀도급상관지표무명현차이（P＞0?05）；자격소결핍시，여OVX조비교，F＋OVX조골량하강경위엄중（P＜0?05）。반정량PCR결과현시：자격소존재시，량조TRAP、CTR、MMP9、cathepsin K기인표체무통계학차이；자격소결핍시， F＋OVX조각기인표체수평교OVX조현저승고（P＜0?05）。거세후ERβ표체하강（P＜0?05），ERα무명현차이。 TRAP염색현시거세전각조소서파골세포수무명현차이（P＞0?05）；이거세후，F＋OVX조파골세포수명현고우OVX조（P＜0?05）。결론미거세시（유자격소），FAC대골흡수영향심미；거세후（무자격소），FAC능현저증강파골활성。
Objective To explore the effect of iron on bone resorption before and after ovariectomy in mice. Methods A mouse model of high iron accumulation was established. The mice were randomly divided into SHAM group, OVX group, and F+OVX group. After 2?week iron intervention, the mice were ovariectomized. Body weight of the mice was examined before and after ovariectomy. Serum ferritin (Fer), gene expression of TRAP, CTR, MMP9, cathepsin K, ERα, and ERβ were detected. The distal femur was analyzed using 3?D micro?CT. Bone marrow?derived cells were extracted from the femurs of each group for osteoclast culturing. Tartrate resistant acid phosphatase ( TRAP) staining was performed to observe the osteoclast differentiation. Results Body weight of the mice between each group was not significantly different. The serum Fer was significantly high in F+OVX group (P<0?05). Micro?CT analysis indicated that BMD and other parameters were not different between SHAM group and iron?treated group in the presence of estrogen (P>0?05). However, BMD in F+OVX group was much lower than that in OVX group (P <0?05). Semi?quantitative RT?PCR showed that the expressions of TRAP, CTR, MMP9, and cathepsin K were not significantly different between the 2 groups in the presence of estrogen. The expressions was increased significantly in F +OVX group than in OVX group ( P <0?05 ) . ERβ significantly decreased after ovariotomy, while the expression of ERα showed no difference. Results of TRAP staining suggested that the number of TRAP positive cells was not different before ovariectomy, but it was higher in F+OVX group than that in OVX group ( P<0?05 ) . Conclusion FAC has little effect on bone resorption in the presence of estrogen. FAC significantly increases osteoclast activity after ovariectomy.