右江民族医学院学报
右江民族醫學院學報
우강민족의학원학보
JOURNAL OF YOUJIANG MEDICAL COLLEGE FOR NATIONALITIES
2015年
1期
5-7,13
,共4页
江旭锋%曾庆春%刘军杰%曾怡%苏莉莉%黄岑汉%刘燕平
江旭鋒%曾慶春%劉軍傑%曾怡%囌莉莉%黃岑漢%劉燕平
강욱봉%증경춘%류군걸%증이%소리리%황잠한%류연평
壮通饮%糖尿病肾病%细胞因子类%荧光实时定量 RT-PCR%mRNA 表达
壯通飲%糖尿病腎病%細胞因子類%熒光實時定量 RT-PCR%mRNA 錶達
장통음%당뇨병신병%세포인자류%형광실시정량 RT-PCR%mRNA 표체
Zhuangtongyin%diabetic nephropathy%cytokines%fluorescent quantitative real-time RT-PCR%mRNA expression
目的:实验性研究壮通饮对糖尿病肾病(DN)大鼠肾皮质结缔组织生长因子(CTGF)、转化生长因子(TGF-β1)、血浆纤溶酶原激活物抑制物(PAI-1)、纤维连接蛋白(FN)细胞因子 mRNA 表达的影响,并探讨壮通饮对 DN 大鼠肾脏的保护作用机制。方法采用单肾切除术合并腹腔注射链脲佐菌素(STZ)诱导制备 DN 大鼠模型,并随机分为空白对照组、假手术组、模型组、卡托普利西药组(1 g/kg)和壮通饮低(6.8 g/kg)、中(13.6 g/kg)、高(27.2 g/kg)剂量7个组。于实验给药治疗6周后,用荧光实时定量 RT-PCR 法检测大鼠肾皮质 CTGF、TGF-β1、PAI-1、FN 细胞因子mRNA 的表达。结果壮通饮可以降低 DN 大鼠肾皮质 CTGF、TGF-β1、PAI-1、FN 细胞因子 mRNA 的表达。结论壮通饮能改善肾皮质 CTGF、TGF-β1、PAI-1、FN 细胞因子 mRNA 的表达,以减轻肾脏病理改变,对 DN 大鼠的肾脏具有一定的保护作用。
目的:實驗性研究壯通飲對糖尿病腎病(DN)大鼠腎皮質結締組織生長因子(CTGF)、轉化生長因子(TGF-β1)、血漿纖溶酶原激活物抑製物(PAI-1)、纖維連接蛋白(FN)細胞因子 mRNA 錶達的影響,併探討壯通飲對 DN 大鼠腎髒的保護作用機製。方法採用單腎切除術閤併腹腔註射鏈脲佐菌素(STZ)誘導製備 DN 大鼠模型,併隨機分為空白對照組、假手術組、模型組、卡託普利西藥組(1 g/kg)和壯通飲低(6.8 g/kg)、中(13.6 g/kg)、高(27.2 g/kg)劑量7箇組。于實驗給藥治療6週後,用熒光實時定量 RT-PCR 法檢測大鼠腎皮質 CTGF、TGF-β1、PAI-1、FN 細胞因子mRNA 的錶達。結果壯通飲可以降低 DN 大鼠腎皮質 CTGF、TGF-β1、PAI-1、FN 細胞因子 mRNA 的錶達。結論壯通飲能改善腎皮質 CTGF、TGF-β1、PAI-1、FN 細胞因子 mRNA 的錶達,以減輕腎髒病理改變,對 DN 大鼠的腎髒具有一定的保護作用。
목적:실험성연구장통음대당뇨병신병(DN)대서신피질결체조직생장인자(CTGF)、전화생장인자(TGF-β1)、혈장섬용매원격활물억제물(PAI-1)、섬유련접단백(FN)세포인자 mRNA 표체적영향,병탐토장통음대 DN 대서신장적보호작용궤제。방법채용단신절제술합병복강주사련뇨좌균소(STZ)유도제비 DN 대서모형,병수궤분위공백대조조、가수술조、모형조、잡탁보리서약조(1 g/kg)화장통음저(6.8 g/kg)、중(13.6 g/kg)、고(27.2 g/kg)제량7개조。우실험급약치료6주후,용형광실시정량 RT-PCR 법검측대서신피질 CTGF、TGF-β1、PAI-1、FN 세포인자mRNA 적표체。결과장통음가이강저 DN 대서신피질 CTGF、TGF-β1、PAI-1、FN 세포인자 mRNA 적표체。결론장통음능개선신피질 CTGF、TGF-β1、PAI-1、FN 세포인자 mRNA 적표체,이감경신장병리개변,대 DN 대서적신장구유일정적보호작용。
Objective This article aims to laboratory study the effects of Chinese herbal Zhuangtongyin on mRNA expression of connective tissue growth factor (CTGF),transforming growth factor (TGF-β1),plas-minogen activator inhibitor 1 (PAI- 1),fibronectin (FN)cytokines in renal cortex of diabetic nephropathy (DN)rats,and to explore the mechanism of Zhuangtongyin in protecting the kidney of DN rats. Methods DN rat models were induced and prepared by mono-nephrectomy combined intraperitoneal injection Streptozo-tocin (STZ),and the rats were randomly divided into 7 groups:a blank control group,a sham operation group,a model group,a western medicine of Captopril group,(1 g/kg)and Zhuangtongyin low dose (6.8 g/kg),medium dose (13.6 g/kg)and high dose (27.2 g/kg)groups.Six weeks after drugs administration,a fluorescent quantitative real-time RT-PCR was used to measure the mRNA expression of CTGF,TGF-β1, PAI-1,FN cytokines in rats renal cortex. Results Zhuangtongyin could down- regulate mRNA expres-sions of CTGF,TGF-β1,PAI-1,FN cytokines in renal cortex of DN rats. Conclusion Zhuantgongyin can protect to a certain extent of DN rats kidney by improving he mRNA expression of CTGF,TGF-β1,PAI-1, FN cytokines in rats renal cortex for lessening the pathological changes of kidney.