CAJ | 학술논문

目的：观察去势联合地塞米松肌肉注射的方法建立大鼠骨质疏松模型的效果。方法将24只3?5月龄雌性SD大鼠（250±20）g随机分成3组，A组：假手术＋肌注生理盐水组（8只）、B组：去势＋肌注生理盐水组（8只）和C组：去势＋肌注地塞米松组（8只）。后两组大鼠行双侧卵巢切除术。分别于术前及术后2w、4w、6w采用（双能X线吸收）骨密度仪进行大鼠全身BMD的测量，术后6w三组大鼠分别进行骨CT值测量、外周血生化检测和股骨颈骨组织形态学观察及其骨小梁形态计量学测定。结果大鼠术后6w， C组全身BMD及骨CT值较A组明显降低（P＜0?05）；B和C组血清Ca浓度较A组明显下降（P＜0?05）；B和C组血清P浓度较A组明显升高（P＜0?05）；B组血清ALP、TRACP浓度较A组明显升高（P＜0?05），C组与B组比较血清ALP浓度显著降低（P＜0?01）、TRACP浓度显著增高（P＜0?01）；B组和C组大鼠骨小梁均变窄，连接不完整出现断裂、局部镂空的现象，骨髓腔变大，骨细胞分布减少，骨小梁形态计量值降低（P＜0?01），C组更明显。结论去势联合肌注地塞米松法建立大鼠骨质疏松症模型较单纯去势法时间短、效果明显、稳定性好。
목적：관찰거세연합지새미송기육주사적방법건립대서골질소송모형적효과。방법장24지3?5월령자성SD대서（250±20）g수궤분성3조，A조：가수술＋기주생리염수조（8지）、B조：거세＋기주생리염수조（8지）화C조：거세＋기주지새미송조（8지）。후량조대서행쌍측란소절제술。분별우술전급술후2w、4w、6w채용（쌍능X선흡수）골밀도의진행대서전신BMD적측량，술후6w삼조대서분별진행골CT치측량、외주혈생화검측화고골경골조직형태학관찰급기골소량형태계량학측정。결과대서술후6w， C조전신BMD급골CT치교A조명현강저（P＜0?05）；B화C조혈청Ca농도교A조명현하강（P＜0?05）；B화C조혈청P농도교A조명현승고（P＜0?05）；B조혈청ALP、TRACP농도교A조명현승고（P＜0?05），C조여B조비교혈청ALP농도현저강저（P＜0?01）、TRACP농도현저증고（P＜0?01）；B조화C조대서골소량균변착，련접불완정출현단렬、국부루공적현상，골수강변대，골세포분포감소，골소량형태계량치강저（P＜0?01），C조경명현。결론거세연합기주지새미송법건립대서골질소송증모형교단순거세법시간단、효과명현、은정성호。
Objective To study the effect of ovariectomy combined with dexamethasone intramuscular injection on the establishment of rat osteoporosis model. Methods Twenty?four 3?5 -month?old female SD rats (250 ± 20 g) were randomly divided into 3 groups: Group A ( sham operation +sodium chloride intramuscular injection ) , Group B ( ovariectomy + sodium chloride intramuscular injection) , and Group C ( ovariectomy+dexamethasone intramuscular injection) . The bilateral ovaries of the last 2 groups were dissected. BMD was measured using dual?energy X?ray densitometry before and 2-, 4-, and 6-week after the operation. Bone CT scan, peripheral blood biochemistry, and the histomorphology of the femoral neck were examined after 6 weeks of the operation. Results In 6 weeks after operation, the whole body BMD and the values of bone CT in group C were lower than that in group A (P<0?05). The serum concentration of Ca in group B and C decreased obviously comparing to that in group A (P<0?05). The serum concentration of P in group B and C increased obviously comparing to that in group A (P<0?05). The serum concentrations of ALP and TRACP in group B increased obviously comparing to that in group A ( P < 0?05 ) . The serum concentration of ALP in group C increased significantly while the TRACP decreased comparing to that in group B (P<0?01). The bone trabecular in group B and C became narrow, uncompleted, ruptured, and even hollowed, while the marrow cavity became larger and the distribution of bone cells and the bone trabecular number decreased ( P<0?01 ) . It was more obvious in group C. Conclusion Ovariectomy combined with dexamethasone intramuscular injection to establish rat osteoporosis model is faster, more effective, and more stabilized than using ovariectomy alone.