CAJ | 학술논문

目的：分析多发性肌炎（PM）患者 FAS／FASL 抗原的表达与外周血淋巴细胞亚群的相关性。方法选取17例我院2012年10月～2014年10月接诊的活动期 PM 患者为研究对象；对照组为非骨骼肌疾病者20例。免疫组化法分别检测两组患者 FAS／FASL 蛋白质在不同部位的表达，流式细胞仪测定两组外周血 CD3＋、CD4＋、CD8＋、CD19 ＋、NK细胞百分率；分析 PM 患者 FAS／FASL 抗原的表达与外周血淋巴细胞亚群的关系。结果结果 FAS、FASL 抗原在 PM肌纤维表面及浸润的淋巴细胞均有较强的表达，与对照组相比，差异有统计学意义（P ＜0．01）；CD4＋、CD8＋ T 细胞百分比、CD4＋／CD8＋的比值高于对照组，差异有统计学意义（P ＜0．05）。结论可能 CD4＋ T 细胞介导细胞免疫与FAS／FASL 蛋白的促进肌细胞凋亡关系更加密切。
목적：분석다발성기염（PM）환자 FAS／FASL 항원적표체여외주혈림파세포아군적상관성。방법선취17례아원2012년10월～2014년10월접진적활동기 PM 환자위연구대상；대조조위비골격기질병자20례。면역조화법분별검측량조환자 FAS／FASL 단백질재불동부위적표체，류식세포의측정량조외주혈 CD3＋、CD4＋、CD8＋、CD19 ＋、NK세포백분솔；분석 PM 환자 FAS／FASL 항원적표체여외주혈림파세포아군적관계。결과결과 FAS、FASL 항원재 PM기섬유표면급침윤적림파세포균유교강적표체，여대조조상비，차이유통계학의의（P ＜0．01）；CD4＋、CD8＋ T 세포백분비、CD4＋／CD8＋적비치고우대조조，차 이 유 통 계 학 의 의（P ＜0．05）。결론가능 CD4＋ T 세포개도세포면역여FAS／FASL 단백적촉진기세포조망관계경가밀절。
Objective To analyze the correlation between the expression of FAS/FASL antigens and pe-ripheral blood lymphocyte subsets in patients with polymyositis (PM). Methods Seventeen patients with ac-tive polymyositis cared at our hospital from October 2012 to October 2014 were involved in this study,and 20 patients with non-skeletal muscle disease were chosen as the controls.An immunohistochemistry method was used to measure the expressions of FAS/FASL proteins at different loci of the two groups,respectively.A flow cytometry instrument was used to detect the peripheral blood lymphocyte subgroup CD3 + ,CD4 + ,CD8 + , CD19 + ,and NK cells percentage.A statistical analysis was performed on the association of the expression of FAS/FASL antigens and peripheral blood lymphocyte subsets in PM patients. Results PM patients were found with higher FAS and FASL antigen expression on PM muscle fibers surface and infiltrating lymphocyte cells,comparing to the control group showed statistically significant difference (P <0.01).The elevated per-centage of CD4+ ,CD8+ T cells and the ratio of CD4+/CD8 + were found in PM group,compared to the control group,there were statistically significant differences (P <0.05). Conclusion Maybe there were more close relationships between the CD4+ T cell mediated cell immuning and FAS/FASL protein in promoting myocyte apoptosis.