CAJ | 학술논문

目的：研究护理干预对大鼠脊髓损伤后脊髓运动功能修复的脊髓形态学变化。方法将60只SD成年大鼠随机分为3组，分别为正常对照组、实验对照组、实验组，每组20只，每组分4个时相点，即脊髓损伤后1 d、7 d、30 d、60 d(n=5)。实验对照组和实验组均采用切割加挤压脊髓L4平面横断制备脊髓损伤大鼠模型。正常对照组为正常大鼠未经处理，实验对照组大鼠脊髓损伤后给予排尿、排便等常规护理，实验组除常规护理之外，还给予关节活动度训练和肌肉按摩训练，每日2次，每次10 min。采用常规HE染色、免疫组织化学染色法观察护理干预对脊髓损伤后大鼠脊髓的形态学变化。结果 HE染色结果以及GFAP和NF-200免疫组织化学染色结果显示，实验组和实验对照组未见明显区别，但与正常对照组比较差异非常明显。结论护理干预对损伤区脊髓组织形态学研究未见明显改变。
목적：연구호리간예대대서척수손상후척수운동공능수복적척수형태학변화。방법장60지SD성년대서수궤분위3조，분별위정상대조조、실험대조조、실험조，매조20지，매조분4개시상점，즉척수손상후1 d、7 d、30 d、60 d(n=5)。실험대조조화실험조균채용절할가제압척수L4평면횡단제비척수손상대서모형。정상대조조위정상대서미경처리，실험대조조대서척수손상후급여배뇨、배편등상규호리，실험조제상규호리지외，환급여관절활동도훈련화기육안마훈련，매일2차，매차10 min。채용상규HE염색、면역조직화학염색법관찰호리간예대척수손상후대서척수적형태학변화。결과 HE염색결과이급GFAP화NF-200면역조직화학염색결과현시，실험조화실험대조조미견명현구별，단여정상대조조비교차이비상명현。결론호리간예대손상구척수조직형태학연구미견명현개변。
Objective To explore the morphology changes of spinal cord after nursing intervention on motor function repair spinal cord injuried rats. Methods 60 adult SD rats were randomly divided into normal control group, experimental control group, and experimental group ( n=20 for each group) . Each group were divided into four time phase points, that is 1 day, 7 days, 30 days and 60 days after spinal cord injury (n=5). The model of L4 plane with full transection of spinal cord were made in the rats in experimental control group and exper-imental group. Normal control group were of untreated normal rats, experimental control group were given routine nursing such as urination and defecation after spinal cord injury, and experimental group were given passive movement practices and muscle massage training twice a day (10 min each time) besides regular nursing. HE staining and immunohistochemistry method were applied to observe the morphology changes of spinal cord. Results In experimental control group and experimental group there was no significant changes in HE staning and NF-200 and GFAP immunohistchemistry staning in spinal cord section of rat at each time phase points, but compared to the normal control group, it was of significant difference. Conclusion There is no apparent change in morphology in injured spinal area after nursing intervention.