CAJ | 학술논문

目的：采用染色法判别日本血吸虫尾蚴的生命状态。方法以0.4%台盼蓝、0.5%M.E.B、0.5%伊红、0.5%甲基蓝、0.05%中性红等5种染色液分别对死活日本血吸虫尾蚴染色5 min，显微镜下观察着色情况。结果台盼蓝、M.E.B、伊红、中性红4种染色液可使死尾蚴着色，而甲基蓝染液对其不着色；5种染液均不能使活尾蚴着色。结论0.4%台盼蓝、0.5%M.E.B、0.5%伊红、0.05%中性红染液染色可判别日本血吸虫尾蚴的生命状态。
목적：채용염색법판별일본혈흡충미유적생명상태。방법이0.4%태반람、0.5%M.E.B、0.5%이홍、0.5%갑기람、0.05%중성홍등5충염색액분별대사활일본혈흡충미유염색5 min，현미경하관찰착색정황。결과태반람、M.E.B、이홍、중성홍4충염색액가사사미유착색，이갑기람염액대기불착색；5충염액균불능사활미유착색。결론0.4%태반람、0.5%M.E.B、0.5%이홍、0.05%중성홍염액염색가판별일본혈흡충미유적생명상태。
Objective To determine the viability of Schistosoma japonicum cercariae by staining. Methods Schistosoma japonicum cercariae were stained by 0.4%trypan blue 0.5%methylene blue?eosin?borax M.E.B 0.5%eosin 0.5%methy?lene blue and 0.05% neutral red respectively for 5 min then they were observed under a stereoscopic microscope. Results The dead cercariae were stained in the trypan blue M.E.B eosin and neutral red but unstained in the methylene blue. The vi?tal cercariae were unstained in all the five kinds of dyes. Conclusion The staining methods by using 0.4% trypan blue 0.5%M.E.B 0.5%eosin and 0.05%neutral red can be used to determine the viability of S. japonicum cercariae.