CAJ | 학술논문

目的：探讨细胞因子TNF-α、IL-6与IL-8在子宫内膜异位症（内异症）的表达及其与盆腔粘连程度的相关性。方法选取子宫内膜异位症患者50例，采用ELISA法分别测定手术前血清、术中腹腔液及术后3个月血清标本TNF-α、IL-6、IL-8含量，并分析与粘连的相关性。结果50例内异症患者中，41例（80.2%）患者合并不同程度的盆腔粘连，内异症患者血清、腹腔液中TNF-α、 IL-6及IL-8显著高于对照组(P<0.05)。内异症Ⅲ～Ⅳ期期患者腹腔液中TNF-α、 IL-6及IL-8的水平显著高于Ⅰ~Ⅱ期，Ⅰ~Ⅱ期Ⅲ~Ⅳ期期患者TNF-α水平显著高于对照组(P<0.05)；术后3个月血清TNF-α、 IL-6及IL-8较术前明显下降（P<0.05）。盆腔粘连程度与腹腔液IL-6、 IL-8、TNF-α均呈正相关（r=0.457， r=0.336， r=0.263， P<0.05）；术前血清细胞因子除IL-8（r=0.135， P>0.05）， TNF-α、 IL-6与粘连呈正相关（r=0.521， r=0.470， P<0.05）。结论细胞因子TNF-α、 IL-6及IL-8与内异症临床分期和治疗疗效密切相关，检测TNF-α、 IL-6及IL-8患者血清的变化，可作为子宫内膜异位症术前评估、疗效评价、复发的重要指标。
목적：탐토세포인자TNF-α、IL-6여IL-8재자궁내막이위증（내이증）적표체급기여분강점련정도적상관성。방법선취자궁내막이위증환자50례，채용ELISA법분별측정수술전혈청、술중복강액급술후3개월혈청표본TNF-α、IL-6、IL-8함량，병분석여점련적상관성。결과50례내이증환자중，41례（80.2%）환자합병불동정도적분강점련，내이증환자혈청、복강액중TNF-α、 IL-6급IL-8현저고우대조조(P<0.05)。내이증Ⅲ～Ⅳ기기환자복강액중TNF-α、 IL-6급IL-8적수평현저고우Ⅰ~Ⅱ기，Ⅰ~Ⅱ기Ⅲ~Ⅳ기기환자TNF-α수평현저고우대조조(P<0.05)；술후3개월혈청TNF-α、 IL-6급IL-8교술전명현하강（P<0.05）。분강점련정도여복강액IL-6、 IL-8、TNF-α균정정상관（r=0.457， r=0.336， r=0.263， P<0.05）；술전혈청세포인자제IL-8（r=0.135， P>0.05）， TNF-α、 IL-6여점련정정상관（r=0.521， r=0.470， P<0.05）。결론세포인자TNF-α、 IL-6급IL-8여내이증림상분기화치료료효밀절상관，검측TNF-α、 IL-6급IL-8환자혈청적변화，가작위자궁내막이위증술전평고、료효평개、복발적중요지표。
Objective To investigate the expression of cytokines TNF-ɑ, IL-6 and IL-8 in endometriosis and its correlation with the degree of pelvic adhesions. Methods 50 patients with endometriosis were selected. The TNF-ɑ, IL-6, IL-8 in serum before operation and 3 months after operation and in peritoneal fluid got intra-operation were detected by ELISA. The correlation with adhesions analyzed. Results 41 cases (80.2%) had different degree of pelvic adhesions, and TNF-ɑ, IL-6 and IL-8 in serum and peritoneal fluid were significantly higher than those in the control group (P<0.05). TNF-alpha, IL-6 and IL-8 in peritoneal fluid in patients with stage Ⅲ~Ⅳ was significantly higher than in those with Ⅰ~Ⅱ stage. The TNF-ɑin patients with stage Ⅰ~Ⅱwas significantly higher than those in the control group (P<0.05);TNF-ɑ, IL-6 and IL-8 in serum 3 months after operation were significantly decreased compared to those before operation ( P<0.05). Pelvic adhesion degree and IL-6, IL-8, TNF-ɑin peritoneal fluid were positively correlated (r=0.457, r=0.336, r=0.263, P<0.05);preoperative serum cytokines except IL-8 (r=0.135, P>0.05), TNF-ɑ, IL-6 was positively associated with adhesions (r=0.521, r=0.470, P<0.05). Conlusion Cytokines TNF-ɑ, IL-6 and IL-8 were closely related to the clinical stage and therapeutic efficacy in the treatment of endometriosis. The change of TNF-ɑ, IL-6 and IL-8 in endometriosis was an important index to preoperative evaluation, therapeutic evaluation and recurrence.