CAJ | 학술논문

利用 ISSR 分子标记技术分析17个蓝莓品种的遗传多样性，并构建 DNA 指纹图谱。从60个 ISSR 引物中筛选出10个稳定的多态性引物。采用 NTSYS-PC 2．0软件进行聚类分析，17个蓝莓品种的遗传相似系数为0．66～0．86，在0．69的水平可分为4个品种群。引物 UBC825、UBC862可将17个蓝莓品种区分开，并依此建立了数字化的 DNA 指纹图谱，为蓝莓品种分类、快速鉴定提供依据。
이용 ISSR 분자표기기술분석17개람매품충적유전다양성，병구건 DNA 지문도보。종60개 ISSR 인물중사선출10개은정적다태성인물。채용 NTSYS-PC 2．0연건진행취류분석，17개람매품충적유전상사계수위0．66～0．86，재0．69적수평가분위4개품충군。인물 UBC825、UBC862가장17개람매품충구분개，병의차건립료수자화적 DNA 지문도보，위람매품충분류、쾌속감정제공의거。
Inter-simple sequence repeat (ISSR)analysis was applied for the determination of the genetic diversity, as well as the establishment of the DNA fingerprints,for 17 selected blueberry cultivars.Ten out of 60 ISSR primers were chosen for the study.Cluster analysis revealed that the genetic similarity coefficients of the 17 samples ranged from 0.66 to 0.86. Using the coefficient threshold of 0.69,4 classifications were formed for the blueberries. They could be positively distinguished by 2 ISSR primers (i.e.,UBC825 and UBC862 ),and used to obtain the digital DNA fingerprints.