宁夏农林科技
寧夏農林科技
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NINGXIA JOURNAL OF AGRICULTURE AND FORESTRY SCIENCE AND TECHNOLOGY
2014年
12期
20-22
,共3页
吴龙军%门惠芹%尹跃%李浩霞%赵建华
吳龍軍%門惠芹%尹躍%李浩霞%趙建華
오룡군%문혜근%윤약%리호하%조건화
枸杞%SRAP%相似系数%亲缘关系
枸杞%SRAP%相似繫數%親緣關繫
구기%SRAP%상사계수%친연관계
Lycium bararum L%SRAP%Similarity coefficient%Phylogenetic relationship
应用相关序列扩增多态性(SRAP)标记,对15个枸杞品种进行了遗传多样性分析。从36对引物组合中筛选8对引物组合用于PCR扩增,共获得39条谱带,多态性条带为37条,平均多态性百分率为94.8%,每对引物组合扩增出条带数3~9条不等,平均每对引物组合得到4.8条。15份材料之间遗传相似系数范围介于0.13~1.00之间,说明供试枸杞品种间存在丰富的遗传多样性。
應用相關序列擴增多態性(SRAP)標記,對15箇枸杞品種進行瞭遺傳多樣性分析。從36對引物組閤中篩選8對引物組閤用于PCR擴增,共穫得39條譜帶,多態性條帶為37條,平均多態性百分率為94.8%,每對引物組閤擴增齣條帶數3~9條不等,平均每對引物組閤得到4.8條。15份材料之間遺傳相似繫數範圍介于0.13~1.00之間,說明供試枸杞品種間存在豐富的遺傳多樣性。
응용상관서렬확증다태성(SRAP)표기,대15개구기품충진행료유전다양성분석。종36대인물조합중사선8대인물조합용우PCR확증,공획득39조보대,다태성조대위37조,평균다태성백분솔위94.8%,매대인물조합확증출조대수3~9조불등,평균매대인물조합득도4.8조。15빈재료지간유전상사계수범위개우0.13~1.00지간,설명공시구기품충간존재봉부적유전다양성。
A genetic diversity analysis was carried out for 15 lycium barbarum varieties by using the sequence related amplified polymorphism (SRAP)marking. Eight pairs of primer combinations were screened from thirty six pairs of primer combinations to be used for PCR amplification,and thirty nine bands were obtained,including thirty seven polymorphic bands,the average polymorphism percentage was 94.8%. The number of polymorphic bands for each primer combination ranged from 3 to 9 bands,and on the average each primer combination gets 4.8 bands. The genetic similarity coefficient varied from 0.13 to 1.00 among 15 lycium barbarum varieties,and the results indicated that there are abundant genetic diversity among lycium barbarum varieties.