中国肝脏病杂志（电子版）
中國肝髒病雜誌（電子版）
중국간장병잡지（전자판）
CHINESE JOURNAL OF LIVER DISEASES(ELECTRONIC VERSION)
2014年
4期
57-62
,共6页
刘娟%许海波%向天新%李细女%邬小萍
劉娟%許海波%嚮天新%李細女%鄔小萍
류연%허해파%향천신%리세녀%오소평
急性肝衰竭%高迁移率族蛋白1%免疫组化%RT-PCR
急性肝衰竭%高遷移率族蛋白1%免疫組化%RT-PCR
급성간쇠갈%고천이솔족단백1%면역조화%RT-PCR
Acute liver failure%High mobility group protein 1%Immunohistochemistry%RT-PCR
目的：探讨HMGB1在急性肝衰竭大鼠肝组织和血中的变化规律及作用。方法将48只健康雌性SD大鼠按数字表随机分为对照组、模型组，每组24只，采用腹腔内注射D-GalN（400 mg/kg）＋LPS （100μg/kg）的方法诱导急性肝衰竭，同时对照组予腹腔注射等量生理盐水。两组大鼠均在4、8、12小时时间点取血液及肝组织标本，检测血清ALT、AST水平，HE染色观察肝组织病理变化，ELISA法检测血清HMGB1浓度，RT-PCR法检测肝组织HMGB1 mRNA表达，免疫组化法检测肝组织HMGB1的表达。结果D-GalN（400 mg/kg）＋LPS（100μg/kg）的方法能成功诱导急性肝衰竭模型。对照组血清HMGB1浓度及肝组织HMGB1 mRNA表达在各时间点均较低，而模型组随时间延长而升高。对照组肝细胞核与胞浆中仅见少量HMGB1蛋白表达，模型组肝组织HMGB1蛋白表达随时间延长升高。结论急性肝衰竭时血和肝组织中HMGB1水平均随时间延长而升高，且两者变化与肝损程度正相关。
目的：探討HMGB1在急性肝衰竭大鼠肝組織和血中的變化規律及作用。方法將48隻健康雌性SD大鼠按數字錶隨機分為對照組、模型組，每組24隻，採用腹腔內註射D-GalN（400 mg/kg）＋LPS （100μg/kg）的方法誘導急性肝衰竭，同時對照組予腹腔註射等量生理鹽水。兩組大鼠均在4、8、12小時時間點取血液及肝組織標本，檢測血清ALT、AST水平，HE染色觀察肝組織病理變化，ELISA法檢測血清HMGB1濃度，RT-PCR法檢測肝組織HMGB1 mRNA錶達，免疫組化法檢測肝組織HMGB1的錶達。結果D-GalN（400 mg/kg）＋LPS（100μg/kg）的方法能成功誘導急性肝衰竭模型。對照組血清HMGB1濃度及肝組織HMGB1 mRNA錶達在各時間點均較低，而模型組隨時間延長而升高。對照組肝細胞覈與胞漿中僅見少量HMGB1蛋白錶達，模型組肝組織HMGB1蛋白錶達隨時間延長升高。結論急性肝衰竭時血和肝組織中HMGB1水平均隨時間延長而升高，且兩者變化與肝損程度正相關。
목적：탐토HMGB1재급성간쇠갈대서간조직화혈중적변화규률급작용。방법장48지건강자성SD대서안수자표수궤분위대조조、모형조，매조24지，채용복강내주사D-GalN（400 mg/kg）＋LPS （100μg/kg）적방법유도급성간쇠갈，동시대조조여복강주사등량생리염수。량조대서균재4、8、12소시시간점취혈액급간조직표본，검측혈청ALT、AST수평，HE염색관찰간조직병리변화，ELISA법검측혈청HMGB1농도，RT-PCR법검측간조직HMGB1 mRNA표체，면역조화법검측간조직HMGB1적표체。결과D-GalN（400 mg/kg）＋LPS（100μg/kg）적방법능성공유도급성간쇠갈모형。대조조혈청HMGB1농도급간조직HMGB1 mRNA표체재각시간점균교저，이모형조수시간연장이승고。대조조간세포핵여포장중부견소량HMGB1단백표체，모형조간조직HMGB1단백표체수시간연장승고。결론급성간쇠갈시혈화간조직중HMGB1수평균수시간연장이승고，차량자변화여간손정도정상관。
Objective To investigate the liver tissue and blood of HMGB1 variation and function in rats with acute liver failure. Methods The 48 healthy female SD rats were randomly divided into control group (n=24), and model group (n=24). D-GalN (400 mg/kg) and LPS (100μg/kg) were uesd to manufacture of acute liver failure model by intraperitoneal injection. Model group was given D-GalN/LPS by intraperitoneal injection, simultaneously, control group was given the same amount of saline. Each groups were taken specimens at 4 h, 8 h and 12 h after ALF induction. Each group was detected for serum ALT and AST concentration, observed liver pathological changes by HE staining, evaluated serum HMGB1 concentration by ELISA assay, and determined the level of HMGB1 mRNA by RT-PCR and the protein expression level of liver tissue HMGB1 by immunohistochemistry. Results The ALF models were successfully induced by D-GalN (400 mg/kg) and LPS (100μg/kg) injection. The serum HMGB1 concentrations and the liver tissue of HMGB1 mRNA expression of control group were lower at all time points, and they increased with time in the model group. Liver nuclei and cytoplasm of the control group only saw a small amount of HMGB1 protein expression in liver tissue, the model group HMGB1 protein expression increased with time. conclusions The blood and liver tissue HMGB1 levels of rats with acute liver failure increased with time, there was a positive correlation between them and the degrees of liver damage.
