CAJ | 학술논문

目的：观察血管紧张素Ⅱ（AngⅡ）对人脐静脉内皮细胞（HUVECs）的致凋亡效应及黄芪含药血清的保护作用。方法将培养的 ECV-304分为以下3组：（1）空白对照组；（2）AngⅡ诱导组，使培养基中 AngⅡ终浓度为0、1×10－6、1×10－5、1×10－4 mol／L，与细胞共孵育18 h。MTT 法检测 AngⅡ对 HUVECs 生长增殖的影响；流式细胞仪检测 AngⅡ作用后内皮细胞凋亡率的变化；电子显微镜观察 AngⅡ诱导后内皮细胞的超微结构特点；（3）黄芪含药血清干预组，培养基中加入不同浓度黄芪含药血清培养24 h 后，加入 AngⅡ（1×10－4 mol／L）孵育18 h，检测内皮细胞凋亡率的变化。结果不同浓度的 AngⅡ均能抑制内皮细胞生长增殖。不同浓度的 AngⅡ均可显著诱导内皮细胞凋亡。透射电镜下可见 AngⅡ诱导后内皮细胞的凋亡形态。黄芪含药血清抑制 AngⅡ所诱导的内皮细胞凋亡。结论黄芪含药血清具有内皮保护作用。
목적：관찰혈관긴장소Ⅱ（AngⅡ）대인제정맥내피세포（HUVECs）적치조망효응급황기함약혈청적보호작용。방법장배양적 ECV-304분위이하3조：（1）공백대조조；（2）AngⅡ유도조，사배양기중 AngⅡ종농도위0、1×10－6、1×10－5、1×10－4 mol／L，여세포공부육18 h。MTT 법검측 AngⅡ대 HUVECs 생장증식적영향；류식세포의검측 AngⅡ작용후내피세포조망솔적변화；전자현미경관찰 AngⅡ유도후내피세포적초미결구특점；（3）황기함약혈청간예조，배양기중가입불동농도황기함약혈청배양24 h 후，가입 AngⅡ（1×10－4 mol／L）부육18 h，검측내피세포조망솔적변화。결과불동농도적 AngⅡ균능억제내피세포생장증식。불동농도적 AngⅡ균가현저유도내피세포조망。투사전경하가견 AngⅡ유도후내피세포적조망형태。황기함약혈청억제 AngⅡ소유도적내피세포조망。결론황기함약혈청구유내피보호작용。
Objective To study AngⅡ induced apoptosis of HUVECs and to observe the protective effect of serum contained huangqi on endothelial cell.Methods ECV-304 cells were randomly divided into control group,AngⅡgroup and huangqi group.In the control group,cells were cultured for 18 h,and the concentration of AngⅡ were 0 mol/L,1×10-6 mol/L,1×10-5 mol/L and 1×10-4 mol/L.The cell proliferation was measured by MTT assay.Electron microscope was used to observe the change of HU-VECs.Ultrastructure of HUVECs induced by AngⅡ was observed by electron microscope.In the huangqi group,serum contained huangqi of different concentration were added into the medium and cultured for 24 h,then AngⅡ of 1×10-4 mol/L was included and cultured for 18 h,and the apoptosis ratio induced by AngⅡwas measured by flow cytometry.Results AngⅡof different con-centration could all significantly inhibit HUVECs proliferation.AngⅡof different concentration could all induce endothelial cell ap-optosis.HUVECs apoptosis was observed by electron microscope.HUVECs apoptosis induced by AngⅡcould be inhibited by ser-um contained huangqi.Conclusion Serum contained huangqi could protect endothelial cells.