CAJ | 학술논문

目的：将 Smad7转染到人骨髓间充质干细胞（BMMSC），并进行绿色荧光标记，将其植入兔青光眼手术模型，观察BMMSC 存活情况。方法采用 BP 反应和 LR 反应，将绿色荧光标记的 Smad7基因片段通过噬菌体插入 BMMSC 中，通过集落筛选，选出阳性表达细胞株。15只新西兰大耳白兔行小梁切除，局部予以 BMMSC，观察细胞存活情况。结果 BMMSC 的Smad7表达稳定，绿色荧光标记成功。BMMSC 在兔活体小梁存活，荧光表达满意，眼压稳定。结论绿色荧光标记的 Smad7能稳定转染到 BMMSC，在兔小梁切除术模型表达稳定。
목적：장 Smad7전염도인골수간충질간세포（BMMSC），병진행록색형광표기，장기식입토청광안수술모형，관찰BMMSC 존활정황。방법채용 BP 반응화 LR 반응，장록색형광표기적 Smad7기인편단통과서균체삽입 BMMSC 중，통과집락사선，선출양성표체세포주。15지신서란대이백토행소량절제，국부여이 BMMSC，관찰세포존활정황。결과 BMMSC 적Smad7표체은정，록색형광표기성공。BMMSC 재토활체소량존활，형광표체만의，안압은정。결론록색형광표기적 Smad7능은정전염도 BMMSC，재토소량절제술모형표체은정。
Objective Human bone marrow mesenchymal stem cells were transfected by Smad7 and labeled with green fluores-cent mark.BMMSCs were implanted into rabbit glaucoma operational model and observed surviving condition.Methods Through BP and LR reaction,Smad7 with green fluorescent mark was inserted into human bone marrow mesenchymal stem cells by bacterio-phage,filtered positive colony and picked out cell line.15 New Zealand white rabbits were enforced trabeculectomy with BMMSC, then following up cell survival condition.Results Smad7 expressed stable in human bone marrow mesenchymal stem cells with sat-isfactory green fluorescent mark.BMMSC survived in rabbit trabecula with stable green fluorescent and effective ocular press.Con-clusion Smad7 with green fluorescent mark could be inserted into human bone marrow mesenchymal stem cells stably,and has ef-fective results in rabbit model.