CAJ | 학술논문

目的：探讨导管分离表皮葡萄球菌的生物被膜形成能力和耐受应激环境能力。方法采用结晶紫半定量法和细菌计数法检测表皮葡萄球菌的生物被膜形成能力和应激环境耐受能力。结果表皮葡萄球菌1457菌株和5株导管分离菌株均具有生物被膜形成能力，菌株之间生物被膜形成能力差异无统计学意义（ P＞0．05）；导管分离菌株与1457菌株的游离细菌和被膜细菌的生长能力、对高分子材料的黏附能力、对过氧化氢的氧化应激耐受能力和对乙醇耐受能力差异无统计学意义（P＞0．05）。结论表皮葡萄球菌导管分离菌株与产生生物被膜的表皮葡萄球菌1457菌株具有相近的生物被膜形成能力和耐受应激环境能力。
목적：탐토도관분리표피포도구균적생물피막형성능력화내수응격배경능력。방법채용결정자반정량법화세균계수법검측표피포도구균적생물피막형성능력화응격배경내수능력。결과표피포도구균1457균주화5주도관분리균주균구유생물피막형성능력，균주지간생물피막형성능력차이무통계학의의（ P＞0．05）；도관분리균주여1457균주적유리세균화피막세균적생장능력、대고분자재료적점부능력、대과양화경적양화응격내수능력화대을순내수능력차이무통계학의의（P＞0．05）。결론표피포도구균도관분리균주여산생생물피막적표피포도구균1457균주구유상근적생물피막형성능력화내수응격배경능력。
Objective To explore the biofilm forming ability and the ability to survive in stress environment of staphylococcus epidermidis strains isolated from catheters .Methods Semiquantitative biofilm assay and bacteria cell counting were performed to explore the biofilm forming ability and the ability to survive in stress environment of staphylococcus epidermidis strains .Results Staphylococcus epidermidis strain of 1457 and 5 clinical strains isolated from catheters had the similar ability of biofilm formation (P>0 .05) ,similar growth ability of planktonic and biofilm cells ,similar attachment ability to polystyrene ,similar ability to survive in an oxidative and ethanol stress environment (P>0 .05) .Conclusion The biofilm forming ability and the ability to survive in stress environment of staphylococcus epidermidis strains isolated from catheters were similar to staphylococcus epidermidis 1457 strain .