CAJ | 학술논문

目的：观察急性胰腺炎（AP）患者外周血单核细胞（PBMCs）Toll样受体9（TLR9）mRNA的表达情况。方法收集52例发病24 h内的AP患者，采集第1、3、5天外周乙二胺四乙酸二钾（EDTAK2）抗凝静脉血，提取血浆低温冻存，用于成批检测胰弹性蛋白酶、促炎细胞因子、抗炎细胞因子等。同法采集其治愈后2～3个月的外周EDTAK2抗凝静脉血行上述检测，作为相应指标的基准水平值。分离外周血单核细胞用于随后成批作逆转录聚合酶链反应（RT‐PCR）检测 TLR9 mRNA的表达变化规律。同法采集其治愈后3个月的外周EDTAK2抗凝静脉血行上述检测，作为相应指标的基准水平值。最终将成功采得治愈后3个月血液标本的前36例患者纳入最终研究。结果 AP患者的外周血PBMCs TLR9 mRNA相对含量高于其痊愈后3个月的基线水平（P＜0．05）。AP患者的外周血PBMCs TLR9 mRNA相对含量表达改变与胰弹性蛋白酶、促炎细胞因子水平呈正相关，与抗炎细胞因子无相关性。结论 AP患者的外周血PBMCs TLR9 mRNA表达显著升高并与促炎细胞因子表达同步上调，提示AP的发生、发展可能通过TLR9介导。
목적：관찰급성이선염（AP）환자외주혈단핵세포（PBMCs）Toll양수체9（TLR9）mRNA적표체정황。방법수집52례발병24 h내적AP환자，채집제1、3、5천외주을이알사을산이갑（EDTAK2）항응정맥혈，제취혈장저온동존，용우성비검측이탄성단백매、촉염세포인자、항염세포인자등。동법채집기치유후2～3개월적외주EDTAK2항응정맥혈행상술검측，작위상응지표적기준수평치。분리외주혈단핵세포용우수후성비작역전록취합매련반응（RT‐PCR）검측 TLR9 mRNA적표체변화규률。동법채집기치유후3개월적외주EDTAK2항응정맥혈행상술검측，작위상응지표적기준수평치。최종장성공채득치유후3개월혈액표본적전36례환자납입최종연구。결과 AP환자적외주혈PBMCs TLR9 mRNA상대함량고우기전유후3개월적기선수평（P＜0．05）。AP환자적외주혈PBMCs TLR9 mRNA상대함량표체개변여이탄성단백매、촉염세포인자수평정정상관，여항염세포인자무상관성。결론 AP환자적외주혈PBMCs TLR9 mRNA표체현저승고병여촉염세포인자표체동보상조，제시AP적발생、발전가능통과TLR9개도。
Objective To investigate the expression of TLR9 mRNA of peripheral blood mononuclear cells (PBMCs) in patients with acute pancreatitis .Methods Fifty two AP patients with the disease duration in 24 h were collected ,peripheral EDTAK2 coag‐ulation vein blood were collected on the first ,third and fifth day ,then plasma were cryop reserved to detect pancreatic elastase , proinflammatory cytokines and anti‐inflammatory cytokines .Then the peripheral EDTAK2 coagulation vein blood two to three months after treatment were collected in the same method to undertake these tests ,and act as the reference level value .Peripheral blood was collected from 36 acute pancreatitis patients .Three months later ,peripheral blood was collected again from these 36 peo‐ple as controls .PBMCs were isolated by Ficoll‐Hypaque gradient centrifugation .RT‐PCR was adopted to determine the relative con‐tent of the expression of TLR9 mRNA of the PBMCs .Results The relative content of expression of TLR9 mRNA were significant‐ly up‐regulated in the patients with acute pancreatitis ,compared with that of controls (P<0 .05) .The up‐regulated expression of TLR9 mRNA was related to expression of TNF‐a and IL‐1 .Conclusion The up‐regulated expression of TLR9 mRNA in acute pan‐creatitis patients indicates that infective factors might be mediated by TLR 9 .