CAJ | 학술논문

目的：探讨食管鳞癌组织和细胞系中Nek8的表达及其与食管鳞癌临床病理特征和术后生存的关系。方法采用RT‐PCR法检测食管鳞癌细胞系Eca109细胞、2例配对的食管鳞癌组织和癌旁黏膜上皮中Nek8 mRNA表达情况；应用免疫组织化学法检测Nek8蛋白在食管鳞癌组织芯片中的表达情况，并结合临床病理资料和生存率进行统计学分析。结果 Nek8 mRNA在Eca109细胞、2例食管鳞癌患者的癌组织均有表达，而癌旁食管黏膜上皮中均未见明显的表达。组织芯片结果示N ek8蛋白主要表达于细胞质，在78例配对的标本中，Nek8在食管鳞癌组织中的表达显著高于其在癌旁黏膜组织的表达（P＝2．16E‐13）。其表达与肿瘤大小有关（P＝0．008），而与性别、年龄、分化程度、浸润深度、淋巴结转移无关（P＞0．05）。生存分析显示高表达和低表达Nek8的患者生存率差异无统计学意义（P＞0．05）。结论 Nek8在食管鳞癌组织和细胞中表达上调，可能参与了食管鳞癌的发生、发展，可作为诊断的标志物和治疗的靶点。
목적：탐토식관린암조직화세포계중Nek8적표체급기여식관린암림상병리특정화술후생존적관계。방법채용RT‐PCR법검측식관린암세포계Eca109세포、2례배대적식관린암조직화암방점막상피중Nek8 mRNA표체정황；응용면역조직화학법검측Nek8단백재식관린암조직심편중적표체정황，병결합림상병리자료화생존솔진행통계학분석。결과 Nek8 mRNA재Eca109세포、2례식관린암환자적암조직균유표체，이암방식관점막상피중균미견명현적표체。조직심편결과시N ek8단백주요표체우세포질，재78례배대적표본중，Nek8재식관린암조직중적표체현저고우기재암방점막조직적표체（P＝2．16E‐13）。기표체여종류대소유관（P＝0．008），이여성별、년령、분화정도、침윤심도、림파결전이무관（P＞0．05）。생존분석현시고표체화저표체Nek8적환자생존솔차이무통계학의의（P＞0．05）。결론 Nek8재식관린암조직화세포중표체상조，가능삼여료식관린암적발생、발전，가작위진단적표지물화치료적파점。
Objective To investigate the expression of Nek8 in esophageal squamous cell carcinoma(ESCC) tissues and cell line , and to evaluate its correlation with the clinicopathological features of ESCC and the survival rate of ESCC patients after operation . Methods The expression of Nek8 mRNA in human ESCC Eca109 cell line and two pairs of ESCC tissues and adjacent normal e‐sophageal mucosal epithelium were detected by semi‐quantitative reverse transcription polymerase chain reaction (RT‐PCR) .Immu‐nohistochemistry and tissue microarray technique were used to examine the expression of Nek 8 protein in ESCC tissues and tumor‐adjacent tissues .The correlation between Nek8 expression and clinicopathological features of ESCC and survival rate of ESCC pa‐tients was then analyzed .Results The expression of Nek8 mRNA was positive in Eca109 cells and two cases of ESCC tissue ,and it was negative in paired normal esophageal mucosal epithelium specimens .In tissue microarray ,the expression of Nek8 protein in ES‐CC tissues ,which was mainly in the cytoplasm ,was significantly higher than that in tumor‐adjacent tissues(P= 2 .16E‐13) .The high expression of Nek8 was associated with tumor size (P=0 .008) ,but not with sex ,age ,histological grade ,infiltration degree , lymph node metastasis ,and the survival rate(P>0 .05) .Conclusion The expression of Nek8 is up‐regulated in ESCC tissues and cell line ,and may be involved in tumorigenesis and development of ESCC .Nek8 could act as a potential biomarker for ESCC diag‐nose and target for therapy .