CAJ | 학술논문

目的：探讨不同染氟剂量在不同时间对雌鼠血细胞的影响。方法选用SD雌性大鼠按体质量分为对照组（饲以非病区玉米饲料，含氟量为5．2mg／kg）、中氟组、高氟组（饲以掺入燃煤型氟中毒病区玉米饲料，含氟量分别为47．8、96mg／kg），中氟组、高氟组合称染氟组。于染氟60、120、180d进行血常规检测。结果染氟60、120d高氟组白细胞（WBC）较中氟组明显减少（P＜0．05）。第60、120天和180天时染氟组红细胞（RBC）较对照组显著减少（P＜0．05），染氟组第120天时RBC较第60、180天减少（P＜0．05）。染氟60、120d时染氟组血红蛋白（Hb）、红细胞压积（HCT）、平均红细胞体积（MCV）较对照组减低（P＜0．05），第180天时染氟组MCV反而有增高趋势（P＜0．05）；染氟组内Hb、HCT染氟120d均比60、180d低（P＜0．05）；中氟组MCV随时间推移逐渐降低，180d时显著低于60d（P＜0．05），高氟组染氟120dMCV均比60、180d低（P＜0．05）。中氟组内染氟120d平均血红蛋白含量（MCH）比60、180d高（P＜0．05），高氟组内染氟180d时MCH比60d高（P＜0．05）。结论氟中毒对SD雌鼠的血细胞有不同程度的影响，尤其对红细胞系统的影响最大。染氟早期、中期RBC表现为小RBC高色素性。染氟晚期表现为大细胞高色素性。
목적：탐토불동염불제량재불동시간대자서혈세포적영향。방법선용SD자성대서안체질량분위대조조（사이비병구옥미사료，함불량위5．2mg／kg）、중불조、고불조（사이참입연매형불중독병구옥미사료，함불량분별위47．8、96mg／kg），중불조、고불조합칭염불조。우염불60、120、180d진행혈상규검측。결과염불60、120d고불조백세포（WBC）교중불조명현감소（P＜0．05）。제60、120천화180천시염불조홍세포（RBC）교대조조현저감소（P＜0．05），염불조제120천시RBC교제60、180천감소（P＜0．05）。염불60、120d시염불조혈홍단백（Hb）、홍세포압적（HCT）、평균홍세포체적（MCV）교대조조감저（P＜0．05），제180천시염불조MCV반이유증고추세（P＜0．05）；염불조내Hb、HCT염불120d균비60、180d저（P＜0．05）；중불조MCV수시간추이축점강저，180d시현저저우60d（P＜0．05），고불조염불120dMCV균비60、180d저（P＜0．05）。중불조내염불120d평균혈홍단백함량（MCH）비60、180d고（P＜0．05），고불조내염불180d시MCH비60d고（P＜0．05）。결론불중독대SD자서적혈세포유불동정도적영향，우기대홍세포계통적영향최대。염불조기、중기RBC표현위소RBC고색소성。염불만기표현위대세포고색소성。
Objective To study the relationship between the complete blood count (CBC) and coal‐buring caused fluorosis of fe‐male rats .Methods Female SD rats were randomly divided into three groups :control group ,medium‐fluorine group and high‐fluo‐rine group .Rats in each exposed group were fed with fodder containing different proportions of corn dried by burning coal from flu‐orosis endemic areas to establish coal‐burning fluorosis model (fluoride of fodder were 47 .8 mg/kg and 96 mg/kg) .The corn of control group′s fodder was collected from non endemic areas (fluoride was 5 .2 mg/kg) .At 60 days ,120 days and 180 days ,the tail vein bloods were analyzed with automated analyzer .Results Compared with medium‐fluorine group ,the WBC of high‐fluorine group decreased at 60 d and 120 d(P<0 .05) .Compared with control group ,the RBC of fluoride treated groups decreased at each time point (P<0 .05) ,especially at 120 d .At 60 d and 120 d ,the Hb ,HCT and MCV decreased(P< 0 .05) .At 180 d ,only the MCV of high‐fluorine group increased obviously(P<0 .05) .At 120 d ,the Hb ,HCT of fluoride treated groups were less than those at 60 d and 180 d (P<0 .05) .The MCV of high‐fluorine group was same as above(P<0 .05) .At 180 d ,the MCV of medium‐fluo‐rine group decreased less than that at 60 d (P< 0 .05) .The MCH of fluoride treated groups increased at each time point (P<0 .05) .At 120 d ,the MCH of medium‐fluorine group increased more than its at 60 d and 180 d (P<0 .05) .At 180 d ,the MCH of higher‐fluorine group increased than those at 60 d (P<0 .05) .Conclusion Fluorosis has varied influence on blood cell of SD rats , especially on red blood cell system .In the early and mid stages ,the coal‐buring caused fluorosis showed the small RBC high pigment anemia .In the late stage ,the coal‐buring caused fluorosis showed the big RBC high pigment anemia .