CAJ | 학술논문

目的：构建大鼠单纯性胰腺创伤模型观察胰腺细胞增殖变化特点并探讨胰腺细胞增殖与组织损伤的关系。方法将60只Wistar大鼠分为2组：撞击组（采用BIM‐Ⅲ生物撞击机构建胰腺创伤大鼠模型，40只）和对照组（假手术组，20只），每组大鼠于建模后6、24、72 h ，7 d处死，采用分光光度法检测各组大鼠血清淀粉酶（AMS）、脂肪酶（LPS）活性，通过TUNEL染色和流式细胞技术测定胰腺组织细胞死亡并分析细胞周期分布特点，Western blot测定胰腺组织Bcl‐2、Bax蛋白的表达。结果撞击组大鼠LPS活性升高时相点晚于AMS且持续时间较长，TUNEL染色、流式细胞检测、Western blot结果揭示胰腺创伤可诱导胰腺组织细胞凋亡和代偿性增生。胰腺细胞增殖变化特点表明胰腺创伤后的最佳治疗时间是发病24 h内。结论同时检测AMS和L PS有助于判定胰腺的外分泌功能受损情况。
목적：구건대서단순성이선창상모형관찰이선세포증식변화특점병탐토이선세포증식여조직손상적관계。방법장60지Wistar대서분위2조：당격조（채용BIM‐Ⅲ생물당격궤구건이선창상대서모형，40지）화대조조（가수술조，20지），매조대서우건모후6、24、72 h ，7 d처사，채용분광광도법검측각조대서혈청정분매（AMS）、지방매（LPS）활성，통과TUNEL염색화류식세포기술측정이선조직세포사망병분석세포주기분포특점，Western blot측정이선조직Bcl‐2、Bax단백적표체。결과당격조대서LPS활성승고시상점만우AMS차지속시간교장，TUNEL염색、류식세포검측、Western blot결과게시이선창상가유도이선조직세포조망화대상성증생。이선세포증식변화특점표명이선창상후적최가치료시간시발병24 h내。결론동시검측AMS화L PS유조우판정이선적외분비공능수손정황。
Objective To study the relationships between tissue damage and the ability of the pancreatic cells to regenerate ,and analyze the alteration of the pancreatic cells regeneration .Methods Sixty rats were divided into two groups :impact group(the pan‐creas was injured by a BIM‐Ⅲ biotical impact machine ,40 rats) and control group(sham operated ,20 rats) .All rats were sacrificed at 6 h ,24 h ,72 h ,7 d after operation .The level of AMS ,LPS in the serum were detected by spectrophotometry ,pancreatic cells re‐generation were examined and analyzed by TUNEL staining and flow cytomertry ,and the Bcl‐2 and Bax expression were measured by Western blot .Results In the impact groups ,LPS was activated later than AMS ,and lasted persistently .The results from TUNEL stain ,flow cytometry and Western blot indicated that pancreatic trauma induces cell death and the compensatory prolifera‐tion of pancreatic cells .The characteristics of pancreatic cells regeneration in the animal model of isolated pancreatic trauma indicate that the proper remedial time is in the first 24h after the pancreatic trauma .Conclusion Detecting AMS and LPS at the same time can help us to determine the exocrine function of pancrease .