重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2015年
4期
436-438
,共3页
程俊%曾晓荣%李鹏云%李妙龄%刘智飞%裴杰%杨艳
程俊%曾曉榮%李鵬雲%李妙齡%劉智飛%裴傑%楊豔
정준%증효영%리붕운%리묘령%류지비%배걸%양염
丹参%丹参酮ⅡA磺酸钠%高效液相色谱法%血清药理学%膜片钳技术
丹參%丹參酮ⅡA磺痠鈉%高效液相色譜法%血清藥理學%膜片鉗技術
단삼%단삼동ⅡA광산납%고효액상색보법%혈청약이학%막편겸기술
salvia miltiorrhita%sodium tanshinoneⅡ-A sulfonate%high efficiency liquid chromatography%serous pharmacology%patch clamp technique
目的:探讨一种将中药血清药理学和电生理学技术结合的方法,并将该方法运用到中药丹参的有效成分丹参酮ⅡA磺酸钠(DS‐201)的舒血管机制研究。方法采用醇提法提取丹参有效成分,将该提取液给予小鼠灌胃,经眼球采血得到小鼠血清,用高效液相色谱法(HPLC)测定小鼠血清中DS‐201的含量,并将该含药血清应用于单通道膜片钳实验,研究其对猪冠状动脉平滑肌细胞(PCASMCs)大电导钙激活钾通道(BKCa )的作用。结果含DS‐201血清浓度线性范围是0.73~1.91μg/mL (r=0.9977),回收率是99.85%~101.47%,血药浓度为(7.32±4.25)μg/mL ;单通道膜片钳实验结果显示小鼠含DS‐201血清对PCASMCs的BKCa有激活作用,但差异无统计学意义(P>0.05)。结论该研究建立的提取丹参有效成分丹参酮的醇提法简单、实用、可靠,且测定DS‐201含量的HPLC方法精确;应选取高质量丹参和(或)提高丹参在体内的生物利用度,才能更好地将血清药理学和电生理学技术结合在一起。
目的:探討一種將中藥血清藥理學和電生理學技術結閤的方法,併將該方法運用到中藥丹參的有效成分丹參酮ⅡA磺痠鈉(DS‐201)的舒血管機製研究。方法採用醇提法提取丹參有效成分,將該提取液給予小鼠灌胃,經眼毬採血得到小鼠血清,用高效液相色譜法(HPLC)測定小鼠血清中DS‐201的含量,併將該含藥血清應用于單通道膜片鉗實驗,研究其對豬冠狀動脈平滑肌細胞(PCASMCs)大電導鈣激活鉀通道(BKCa )的作用。結果含DS‐201血清濃度線性範圍是0.73~1.91μg/mL (r=0.9977),迴收率是99.85%~101.47%,血藥濃度為(7.32±4.25)μg/mL ;單通道膜片鉗實驗結果顯示小鼠含DS‐201血清對PCASMCs的BKCa有激活作用,但差異無統計學意義(P>0.05)。結論該研究建立的提取丹參有效成分丹參酮的醇提法簡單、實用、可靠,且測定DS‐201含量的HPLC方法精確;應選取高質量丹參和(或)提高丹參在體內的生物利用度,纔能更好地將血清藥理學和電生理學技術結閤在一起。
목적:탐토일충장중약혈청약이학화전생이학기술결합적방법,병장해방법운용도중약단삼적유효성분단삼동ⅡA광산납(DS‐201)적서혈관궤제연구。방법채용순제법제취단삼유효성분,장해제취액급여소서관위,경안구채혈득도소서혈청,용고효액상색보법(HPLC)측정소서혈청중DS‐201적함량,병장해함약혈청응용우단통도막편겸실험,연구기대저관상동맥평활기세포(PCASMCs)대전도개격활갑통도(BKCa )적작용。결과함DS‐201혈청농도선성범위시0.73~1.91μg/mL (r=0.9977),회수솔시99.85%~101.47%,혈약농도위(7.32±4.25)μg/mL ;단통도막편겸실험결과현시소서함DS‐201혈청대PCASMCs적BKCa유격활작용,단차이무통계학의의(P>0.05)。결론해연구건립적제취단삼유효성분단삼동적순제법간단、실용、가고,차측정DS‐201함량적HPLC방법정학;응선취고질량단삼화(혹)제고단삼재체내적생물이용도,재능경호지장혈청약이학화전생이학기술결합재일기。
Objective To discuss a method combining serum‐pharmacology and electrophysiology technology ,and to research the mechanism of dilating porcine coronary artery of sodium tanshinone Ⅱ‐A sulfonate (DS‐201) .Methods To give mice intragastric administration solution and measure DS‐201 concentration in mice serum ,and apply the serum to single channel patch to research its effect on big conductance calcium‐activated potassium channels(BKca ) in porcine coronary artery smooth muscle cells (PCASMCs) . Results The linear range of concentration containing DS‐201 serum was 0 .73 to 1 .91 μg/mL (r=0 .997 7) ,the recycle rate was 99 .85% -101 .47% ,and the concentration was(7 .32 ± 4 .25)μg/mL ;the result indicates that serum containing DS‐201 has activa‐tion effects on BKCa in PCASMCs ,while there was no statistical significance (P>0 .05) .Conclusion The establishment method of the alcohol extraction of Danshen is useful and reliable .The HPLC method of measuring DS‐201 concentration is precise .Choosing higher quality drugs or raising bioavailability can improve combination of the serum pharmacology and electrophysiological tech‐nique .